偏钒酸铵对四氧嘧啶毒性小鼠空肠组织结构的影响  

Effect of Ammonium Metavanadate on Alloxan Toxicity Mice Jejunum Tissue Structure

作　　者：周金星[1] 靳二辉[1] 李升和[1] 冯保明[1] 潘超[1] 

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2012年第6期14-18,共5页Journal of Anhui Science and Technology University

基　　金：安徽省教育厅自然科学基金项目(KJ2012Z065)

摘　　要：目的:研究偏钒酸铵对四氧嘧啶毒性小鼠空肠组织结构的影响。方法:取重量均在30g左右的小鼠60只,分成A、B、C、D、E五组,每组12只,其中A、B、C组为试验组,D组为模型组,E组为空白对照组,适应性饲喂一周后,按200mg/kg的剂量腹腔注射2.5%四氧嘧啶生理盐水,24h后,A、B、C组分别饮饲20mg/L、40mg/L、80mg/L的偏钒酸铵溶液,D组和E组正常饮水。饲水8d后,断脊处死后取空肠标本制作石蜡切片,HE染色,显微观察、摄影。结果:模型组小鼠空肠绒毛变短,部分上皮细胞脱落,肠腺变小且密度减少。饮饲20mg/L的偏钒酸铵溶液时,空肠绒毛和肠腺的组织结构较完整。饮饲40mg/L的偏钒酸铵溶液时,空肠绒毛指状突起结构清晰,排列整齐,肠腺较多。饮饲80mg/L的偏钒酸铵溶液时,空肠绒毛和肠腺的组织结构受到损害。结论:2.5%四氧嘧啶溶液对小鼠的空肠具有毒性作用,而20mg/L和40mg/L偏钒酸铵对其组织结构有一定的保护作用,其中40mg/L最为显著。Objective：To study the effect of ammonium metavanadate on alloxan toxicity mice jejunum tissue structure. Methods ：60 SD mice were randomly divided into A, B, C, D and E groups, 12 in each group. Among them A, B and C groups are for experimental groups, D group is for model group, and E group is for control group. Feed one week for adaptability and then weigh rats and according 200mg/kg intraperitoneal injection 2.5% alloxan, after 24h, add different vanadium 20,40,80mg/L separately in each kilogram potable water in A,B,C, the group D and the control group use normal drinking water. After 8 days with drinking water, kill all mice and take the jejunum and made into paraffin section, HE dies, Microscope observation and photography. Results Mice jejunum villi shorten in model group, part of epithelial ceils fall off, intestinal gland smaller and it＇ s densily decreased. When feeding 20mg/L am- monium metavanadate, the structure of villus and intestinal glands were complete relatively. When feeding 40mg/L ammonium metavanadate, the structure of jejunum villi fingerlike was clear, arrangement regularity, number of intesti- nal gland was more. When feeding 80mg/L ammonium metavanadate, the structure of villus and intestinal glands were damaged. Conclusion The jejunum of mice could be poisoned by 2.5% alloxan liquor, and the possible alleviation of alloxan toxicity in the jejunum tissue structure increases by 20mg/L and 40mg/L ammonium metavanadate liquor, then 40mg/L was more prominent.

关 键 词：四氧嘧啶 偏钒酸铵 空肠 小鼠 

分 类 号：R994.6[医药卫生—毒理学]