不同温度对马鹿精子活力的影响  

Effects of Different Temperature on Sperm Motility of Wapiti

作　　者：骆新荣[1,2] 费坤学[3] 林敏[4] 周伟平[5] 高庆华[1,2] 

机构地区：[1]新疆塔里木大学动物科学学院,阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室,阿拉尔843300 [3]新疆生产建设兵团农二师25团,库尔勒841403 [4]新疆生产建设兵团农二师33团,库尔勒841403 [5]新疆生产建设兵团农二师31团,库尔勒841403

出　　处：《经济动物学报》2012年第4期192-194,共3页Journal of Economic Animal

基　　金：兵团科技计划项目(2011BD050);塔里木大学校长基金创新群体项目(TDZKCX201201)

摘　　要：为优选马鹿精子在流式分选前保存的方法,对6头种用马鹿麻醉电刺激采精,用改良的TRIS液稀释至精子含量为109个/mL,均分成两份,一份降温到19℃并保存18 h,另一份降温至0℃,在该温度下保存16 h,每隔2 h检测精子活率。结果表明:4 h内19℃保存条件下精子活率显著高于在0℃的,而4 h以后,在0℃保存条件下精子活率显著高于19℃的。To select the best way for storage of wapiti sperm before flow cytomeric sorting, the semen was collected by electro-ejaculation from six wapiti of proven fertility. Ejaculates were diluted to the concen- tration of 1 x 109 sperm/mL with one modified TRIS extender and divided into two groups. One group was cooled to 19 ℃ and maintained at that temperature, and another was cooled to 0 ℃ and maintained at that temperature, too. The sperm motility of two groups was immediately assessed and recorded every 2 h. The sperm motility of the 19 ℃ group was significantly higher than that of the 0 ℃group in 4 h. Four hours later, the result was contradictory（opposite）.

关 键 词：马鹿 精子 保存方法 

分 类 号：S864.3[农业科学—野生动物驯养]