SDF-1α促进骨髓间充质干细胞在脑缺血／再灌注大鼠海马中的存活与分化  

The survival and differentiation of bone marrow mesenchymal stem cells （BMSCs） mediated by SDF - 1α in cerebral ischemia/reperfusion rat hippocampus

作　　者：王玉兰[1,2] 李爱娜[1] 张世春[1] 刘志安[1,2] 高殿帅[2] 徐铁军[1,2] 

机构地区：[1]徐州医学院解剖学教研室,江苏徐州221004 [2]徐州医学院神经生物学教研室

出　　处：《徐州医学院学报》2012年第12期799-802,共4页Acta Academiae Medicinae Xuzhou

基　　金：基金项目：国家自然科学基金（81171141）;徐州医学院院长专项人才基金（2010KJZ19）

摘　　要：目的观察基质细胞衍生因子1α（SDF-1α）对脑缺血／再灌注大鼠海马中移植的骨髓问充质干细胞（BMSCs）的作用。方法培养成年大鼠BMSCs并用BrdU标记，“四血管阻断法”制备大鼠脑缺血／再灌注模型。造模成功大鼠复灌24h后分为模型对照组、BMSC组和SDF-1α＋BMSC组。后两组经尾静脉分别注射BMSCs或SDF-1α＋BMSCs，模型对照组同法注射等体积生理盐水。分别于注射后存活1、3、7天和1个月时，应用免疫荧光化学法检测大鼠海马区域BrdU标记阳性细胞的表达情况。结果免疫荧光染色显示：①模型对照组大鼠在移植不同时间点海马结构中均未见BrdU标记的阳性细胞，但BMSC组和SDF-1α＋BMSC组的BrdU标记阳性细胞数随移植时间延长而逐渐增多，且各时间点问差异均有统计学意义（P〈0．05）；②SDF-1α＋BMSC组移植后1、3、7天BrdU阳性细胞数均显著高于相应时间点的BMSC组（P〈0．05），其中7天时的差异最显著（P〈0．01），但1个月时两者差异无统计学意义（P〉0．05）。结论SDF—1α可显著促进BMSCs在脑缺血／再灌注大鼠海马中的存活与分化，提示SDF-1α在BMSCs移植治疗缺血性脑损伤过程中可起到促进作用。Objective To investigate the effect of bone marrow mesenchymal stem cells （BMSCs） mediated by SDF - 1α at different time points after transplanted BMSCs in rat hippocampus subjected to cerebral ischemia/reperfusion （I/ R）. Methods Cultured BMSCs were labeled by the BrdU and ＂four vessel occlusion＂ method were used to estahlish cerebral I/R model. The models were divided into three groups： control group, BMSCs group and SDF - 1α ＋ BMSCs group. After reperfusion for 24 h, the normal saline, BMSCs and SDF - 1α ＋ BMSCs were transplanted into the I/R mod- el by the tail vein respectively, and immunofluorescence determination was employed for examining the BMSCs of migra- ting to hippocampus at different time points of transplanting the BMSCs. Results Immunofluorescence staining showed that there was no positive cell of BMSCs labeled BrdU （ BrdU ＋ - BMSCs ） in the hippocampus of the control group; Br- dU ＋ - BMSCs were found in the hippocampus of BMSCs group and SDF - 1α ＋ BMSCs group at transplanting different time points, and the number of positive cells were increased following transplanting time ; and the number of positive cells were significant difference at transplanting 3 d, 7 d and 1 M hippocampus compared with 1 d （P 〈 0.05 ）. Compared with BMSCs group, the number of positive cell had significant difference at transplanted 7 d of SDF - 1α ＋ BMSCs group in hippocampus （P 〈 0.05）, but there was no statistical difference at 1 M group （P 〉 O. 05）. Conclusion The SDF - 1α can promote the survival and differentiation of BMSCs, and these results suggest that the SDF - 1α plays a stimulative role during the BMSCs treats the ischemic cerebral injury.

关 键 词：基质细胞衍生因子1Α 骨髓间充质干细胞 缺血 再灌注 海马 大鼠 

分 类 号：R338.2[医药卫生—人体生理学] R741.02[医药卫生—基础医学]