板栗苞壳提取物对思茅松毛虫肠道细菌的抑菌作用  

Inhibition effect of Castanea mollissima Blume bract extract on intestinal bacteria in Dendrolimu kikuchii Matsumura

作　　者：刘绍雄[1] 王明月[1] 王金华[1] 张敬宜[1] 思斯[1] 熊智[1] 

机构地区：[1]西南林业大学/西南山地森林资源保育与利用省部共建教育部重点实验室,昆明650224

出　　处：《南方农业学报》2012年第12期1968-1972,共5页Journal of Southern Agriculture

基　　金：云南省自然科学基金面上项目(2009CD073);云南省中青年学术技术带头人后备人才培养项目(2010CI016);云南省森林灾害预警与控制重点实验室开放基金项目(ZK09A102);西南林业大学大学生创新基金项目(1138;1146);西南林业大学生物技术特色专业建设项目(50116001)

摘　　要：【目的】研究板栗苞壳提取物对思茅松毛虫肠道细菌的抑菌作用,为害虫防治及新型植物源生物农药开发提供理论依据。【方法】以10株思茅松毛虫3龄和5龄幼虫肠道细菌为供试菌种,采用牛津杯法测定不同有机溶剂板栗苞壳提取物的抑菌效果。【结果】不同有机溶剂板栗苞壳提取物对思茅松毛虫肠道细菌均有一定的抑菌作用,且随着质量浓度的增大抑菌作用逐渐增强,各提取物的抑菌作用大小顺序为乙酸乙酯>乙醇>氯仿>正丁醇>石油醚,其中以乙酸乙酯提取物对思茅松毛虫肠道细菌的抑菌作用最强,最低抑菌质量浓度(MIC)为6.250mg/mL,石油醚提取物的抑菌作用不明显或无抑菌作用。【结论】板栗苞壳提取物可作为新型植物源生物农药在思茅松毛虫防治中加以开发利用。[Objective]This research studied the inhibition effect of Castanea mollissima Blume bract extract on in- testinal bacteria in Dendrolimu kikuchff Matsumura in order to create new biological pesticides developed from plant sources and to provide theoretical basis for pesticide development. [Method]Using 10 strains of intestinal bacteria extracted from age 3 and age 5 Dendrolimu kikucbff Matsumura instar larvae as test strains, the inhibition effect of different organic Castanea mollissima Blume bract extract solvents was determined using the Oxford cup method. [Result]Different organic solvents of Castanea mollissima Blume bract extract had some inhibition effects on intestinal bacteria in Dendrolimu kikuehff Matsumura. As the quality concentration increased, the inhibition effect became more noticeable; different ex- tractions were arranged in the following rank from the strongest inhibition rate to the weakest inhibition rate： acetic ether〉 ethanol〉chloroform〉butyl alcohol〉light petroleum. Ethyl acetate extract exhibited strong inhibition activity on the intestinal bacteria of Dendrolimu kikuchii Matsumura. The minimum inhibitory concentration （MIC） was 6.250 mg/mL, and the in- hibition effect of petroleum ether extract was not obvious or non-existent. [ Conclusion ] Castanea mollissima Blume bract extract could be developed as the new plant biological source pesticide to control Dendrolimu kikuchii Matsumura.

关 键 词：板栗苞壳 提取物 思茅松毛虫 肠道细菌 抑菌作用 

分 类 号：S763.42[农业科学—森林保护学]