半滑舌鳎养殖群体性腺发育观察  

Gonadal development process observation of half-smooth tongue sole in rearing population

作　　者：梁卓[1,2] 陈松林[1] 张静[1,2] 宋文涛[1,2] 刘姗姗[1,2] 

机构地区：[1]中国水产科学研究院黄海水产研究所/农业部海洋渔业资源可持续利用重点开放实验室,山东青岛266071 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《南方农业学报》2012年第12期2074-2078,共5页Journal of Southern Agriculture

基　　金：国家"863"计划项目(2012AA10A403-2);国家公益性行业(农业)科研专项项目(200903046);山东省泰山学者工程专项项目(2007-2012)

摘　　要：【目的】了解半滑舌鳎养殖群体的性别遗传比例及雌、雄性腺发育过程,为高比例雌性育种提供参考依据。【方法】利用性别连锁微卫星标记scaffold 1128_343检测两个半滑舌鳎养殖群体生长过程中雌性比例的变化特征,并结合遗传性别鉴定和石蜡切片技术,跟踪观察半滑舌鳎25日龄至两年性成熟的整个性腺发育过程。【结果】对12～76日龄的两个养殖群体进行遗传性别鉴定,发现群体I和群体II的平均遗传雌性比例分别为50.00%和50.23%,基本符合1∶1的分布规律。半滑舌鳎性腺发育早期(25日龄)雌鱼和雄鱼的性腺结构无明显差别,至56日龄雌、雄鱼性腺结构开始出现差别,至76日龄雌、雄鱼性腺结构出现明显差别,至106日龄雌、雄鱼性腺出现明显分化,可通过性腺切片鉴定出雌、雄性别。【结论】半滑舌鳎雌、雄鱼遗传性别比例基本符合1∶1的分布规律,雌鱼性腺发育和分化较雄性更快、过程更复杂。[Objective]This research aimed to understand the cultured genetic gender proportion and the female vs. male gonadal development process of Cynogiossus semilaevis groups in order to provide reference for high proportion fe- male breeding. [Method ]The sex linkage microsatellite markers scaffold1128_343 was used to detect the juvenile female proportion variation characteristics of the two groups of half-smooth tough sole; combined with the genetic sex identifica- tion and paraffin section technique, the gonad sexual maturity development was also observed through tracking the two groups from 25 days old to 2 years old. [Result]From the results of genetic sex identification performed on the two breeding groups of half--smooth tough sole aged 12 to 76 days,the average genetic female percentage of group I and group II was re- spectively 50.00 and 50.23%, which coincided with the 1：1 distribution. The female and male gonadal structure of early half- smooth tough sole gonad development （25 days old） had no significant difference, but starting from the age of 56 days, the female and male gonadal structure started to display visible differences. At 76 days of age, the female and male gonadal structure showed obvious visible differences. At 106 clays of age, the female and male gonads appeared to be obviously dif- ferentiated, which, though the gonadal biopsy meothd, the female and male gender could be identified. [Conclusion]The female vs. male ratio of Cynoglossus semilaevis accorded with the 1：1 distribution. The female gonad development and dif- ferentiation were faster than those of the male gonad development, but the development processes were more complex.

关 键 词：半滑舌鳎 性腺发育 遗传性别鉴定 石蜡切片 

分 类 号：S917.4[农业科学—水产科学]