心房快速起搏致豚鼠房颤模型建立及心房肌动作电位的改变  

The establishment of AF model of guinea pig by atrial rapid pacing and the observation of the changes of action potential duration in the AF model

作　　者：陈唐葶[1] 李涛[1] 李妙龄[1] 刘智飞[1] 杨艳[1] 曾晓荣[1] 

机构地区：[1]泸州医学院心血管医学研究所医学电生理实验室,四川泸州646000

出　　处：《泸州医学院学报》2012年第6期551-553,共3页Journal of Luzhou Medical College

基　　金：国家自然科学基金项目(30870903);四川省科技厅支撑计划(2011FZ0106);四川省教育厅创新团队项目(川教函[2010]597)

摘　　要：目的:建立一种快速高效的豚鼠急性心房颤动(atrial fibrillation,AF)模型,探讨房颤对心房肌动作电位时程(APD)的影响。方法:高频刺激豚鼠右心房处诱发房颤,记录心电图(ECG)及单相动作电位(MAP)的改变。结果:6只豚鼠共给予Burst刺激70次,以AF诱发时长>1s为模型建立成功。实验共诱发AF成功62次,成功率为88.75%,平均时长为9.860±22.035s。模型建立成功的豚鼠心房率显著增加,由205.184±31.278次/min增加到661.312±251.401次/min(P<0.05)。心房肌APD10、APD20、APD50、APD90及P-P周期长度(CL)在AF发作时显著减小(P<0.05),分别由6.038±4.172ms、13.029±11.003ms、37.193±12.504ms、84.453±24.567ms、300.148±52.020ms减小到3.291±1.906ms、6.451±3.140ms、18.227±12.646ms、51.887±36.137ms、105.414±44.567ms。动作电位幅值由AF前的34.422±10.425mV降至AF时的15.285±7.499mV,显著降低(P<0.05)。结论:经高频刺激右心房方法制作房颤动物模型成功率高、重复性好,并伴有心房肌的电生理改变,是建立急性房颤模型的有效方法。Objective：To establish an efficient acute atrial fibrillation（AF） model and observe the changes of action potential duration（APD） in AF.Methods：Atrial fibrillation was induced by rapid pacing the right atrium of guinea pigs,and the electrocardiogram（ECG） and monophasic action potential（MAP） was recorded.Results： 70 times rapid pacing in 6 guinea pigs were used as stimulation,and the durations of AF 1s were defined as success.AF models were induced 62 times through Burst stimulation,with 88.75% success.The guinea pig atrial rate increased significantly,from 205.184±31.278 times/min to 661.312±251.401 times/min（P〈0.05）.During AF,the APD10,APD20,APD50,APD90 and cycle length（CL） significantly decreased（P0.05）,and action potential amplitude significantly reduced（P〈0.05）.Conclusion：The acutely rapid pacing – induced AF model is an effective method for the study of electrophysiological changes of atrial myocardium with high successful rate and repeatability.

关 键 词：心房颤动 动物模型 单相动作电位 豚鼠 

分 类 号：R332[医药卫生—人体生理学] R541.75[医药卫生—基础医学]