解聚复肾宁对糖尿病鼠尿TGF-β1的影响及肾保护作用研究  

Effect of Jiejufushenning on transforming growth factor-beta 1 and kidney function of diabetic rats

作　　者：陈安凤[1] 曹文富[2] 

机构地区：[1]江油市人民医院中西医结合科,四川江油621700 [2]重庆医科大学附属第一医院中西医结合科

出　　处：《泸州医学院学报》2012年第6期598-600,共3页Journal of Luzhou Medical College

摘　　要：目的:探讨解聚复肾宁(JJFSN)对糖尿病(DM)大鼠尿转化生长因子-β1(TGF-β1)的影响及肾脏保护作用。方法:建立链脲佐菌素(STZ)诱导的SD大鼠DM模型,将成模DM大鼠随机分为2组:DM模型(B组)、JJFSN组(C组),另设正常对照组(A组)。采用相应干预措施处理12周。常规方法测定12周末各组大鼠肾功能、24h尿量、24h尿蛋白定量(24hPro)、尿肌酐;ELISA法测定尿TGF-β1的水平;光镜观察肾脏形态。结果:与DM模型比较,JJFSN组大鼠尿素氮(BUN)、血肌酐(Scr)、24hPro、24h尿量、尿TGF-β1水平明显降低(P﹤0.05),尿肌酐排泄增高(P﹤0.05)。结论:JJFSN对DM大鼠肾脏具有保护作用,其机制可能与降低尿TGF-β1有关。Objective： To investigate the effect of Jiejufushenning on urine transforming growth factor-beta 1 and kidney function of diabetic rats.Methods： Diabetic rats induced by injection of streptozotocin（STZ） were randomly divided into diabetic control group（group B）,and Jiejufushenning group（group C）,age-matched normal SD rats served as normal control group（group A）.After 12 weeks of treatment,renal function,urinary volume 24h Pro and creatinine determined by normal methods,TGF-β1 was determined by ELISA method,and the morphological structure of renal tissue was observed by light microscope.Results： Compared with group B,the levels of wrine BUN,Scr,24hPro,volume and TGF-β1 obviously decreased（P〈0.05）,and the excretion of urine creatinine obviously increased in group C（P〈0.05）.Conclusion： Jiejufushenning has the protecting effect on kidney of diabetic rats;the mechanism might be related to the decreasing level of TGF-β1 in urine.

关 键 词：解聚复肾宁 糖尿病大鼠 转化生长因子-Β1 

分 类 号：R587.1[医药卫生—内分泌]