藏药细果角茴香醇提物对内毒素炎症小鼠的保护作用  

Protection effect of herb of thinfruit hypecoum(Xì Guǒ Jiǎo Huí Xiānɡ) alcohol extract on endotoxin induced inflammatory mice

作　　者：刘晓娟[1] 魏红 杨娇[3] 俞碧云[4] 季玉红[5] 

机构地区：[1]南通大学医学院病原生物学系,江苏南通226001 [2]新疆自治区人民医院北院儿科,新疆乌鲁木齐830054 [3]南通大学医学院免疫学实验室,江苏南通226001 [4]南通大学神经再生重点实验室,江苏南通226001 [5]南通大学医学院免疫学教研室,江苏南通226001

出　　处：《苏州大学学报（医学版）》2012年第6期754-759,共6页Suzhou University Journal of Medical Science

基　　金：国家科技支撑计划项目(2007BAI45B00);安徽省高校优秀青年人才基金项目(0470141027)

摘　　要：目的探讨藏药细果角茴香醇提物对革兰阴性细菌的内毒素即脂多糖(LPS)所致小鼠全身炎症反应的保护作用。方法应用BALB/c小鼠腹腔注射LPS导致全身炎症模型,观察小鼠内毒素炎症模型中,细果角茴香醇提物对小鼠肺组织炎症反应和炎性细胞浸润情况、外周血白细胞计数及血清肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的影响。结果细果角茴香醇提物减少内毒素炎症过程中肺组织炎症反应和炎性细胞的浸润,降低外周血白细胞数,抑制TNF-α、IL-6的释放(均P<0.05)。结论细果角茴香醇提物通过多种途径发挥抗炎作用。Objective To study the protection effect and related mechanism of Herb of Thinfruit Hypecoum alcohol extract on endotoxin induced inflammatory mice model. Methods BALB/c mice were administrated with LPS intraperitoneal injection to perform systemic inflammation models, then were given Herb of Thinfruit Hypecoum alcohol extract by gavage. The inflammatory response in lung tissue, inflammatory cells alveolar infiltration, white blood cells （WBC） count in peripheral blood, tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） concentration in sera were measured by HE stain, flow cytometry, ELISA, respectively. Results Herb of Thinfruit Hypeeoum alcohol extract showed anti- inflammatory effect by repressing the inflammatory progress initiated by endotoxin in multiple pathways, including reducing inflammatory response and inflammatory cells infiltration in lung tissue, down- regulating the increasing leukocytes count in peripheral blood and decreasing TNF-α and IL-6 concentration in sera （ all P 〈 0. 05 ）. Conclusion Herb of Thinfruit Hypeeoum can attenuate inflammatory injury via diverse pathways.

关 键 词：藏药 细果角茴香 脂多糖 TNF-Α IL-6 

分 类 号：R291.408[医药卫生—民族医学]