急性β射线皮肤损伤创面愈合过程中相关凋亡基因的表达  

Expression of apoptosis-related genes in acute β-irradiated skin injury in rats

作　　者：赵小瑜[1] 何罕亮[1] 祁强[1] 林伟[1] 沈国良[1] 

机构地区：[1]苏州大学附属第一医院烧伤整形外科,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2012年第6期853-857,878,共6页Suzhou University Journal of Medical Science

基　　金：苏州市科技发展计划(应用基础研究)基金资助项目(SYS201019)

摘　　要：目的研究大鼠急性β射线皮肤损伤创面愈合过程中相关凋亡基因Bcl-2、Bax和P53表达的动态变化,探讨急性β射线皮肤损伤创面难以愈合的机制。方法雌性SD清洁级大鼠54只,随机分为3组:照射组(n=24)为急性深Ⅱ度β射线皮肤损伤动物模型,烫伤组(n=24)为深Ⅱ度热力烫伤动物模型,对照组(n=6)为正常大鼠。取不同时期创面组织,采用原位末端标记(TUNEL)、免疫组织化学等方法检测不同时相点各组大鼠创面局部相关凋亡基因Bcl-2、Bax和P53的变化。结果 (1)TUNEL结果显示,照射组大鼠创面出现后0、1、2、3周的积分吸光度(IA)值明显高于烫伤组,差异有统计学意义(P<0.05)。(2)免疫组化检测结果显示,照射组创面出现后0、1、2、3周Bax、P53的IA值明显高于烫伤组,差异有统计学意义(P<0.05);而烫伤组Bcl-2的IA值明显高于照射组,差异有统计学意义(P<0.05)。结论急性β射线皮肤损伤创面细胞凋亡出现较早、频度较高、消失推迟,可能是导致创面难以愈合的重要原因之一。Objective To investigate the dynamic expression of apoptosis-related genes Bcl-2, Bax and P53 in acute radiation-induced skin ulcers, and to explore the underlying mechanism involved in re- tarded healing of the ulcer. Methods Fifty-four female SD rats were divided into 3 groups. The model of acute radiation-induced skin injury in rats was replicated with 45 Gy electron accelerator β-ray to the skin as radiation group （ n = 24） ; the model of deep second degree scald in rats was established as burn group （ n = 24） ; 6 normal rats were served as normal control group. From different periods skin wounds, the ex- pression of Bcl-2, Bax and P53 were respectively assessed by means of immunohistochemical technique and apoptosis was observed by TUNEL assay. Results （ 1 ） The result of the TUNEL manifested that the integral absorbance （IA） of the radiation group was much higher than that of the control group. There is statistical significance between the two groups （P 〈 0.05 ）. （2） O, 1, 2, 3 weeks after wound emerging, the Bax and P53 integral absorbance （IA） in radiation group was much higher than that of the control group. The Bcl-2 intergral absorbance （IA） in burn group was much higher than that of the radiation group. There is statistical significance between the two groups （P 〈 0.05 ）. Conclusions It was shown that apoptosis of β radiation manifested three typical characteristics, namely early occurrence, high fre- quency and delayed disappearance after radiation, which might explain the delayed wound healing caused by β radiation.

关 键 词：Β射线 放射性皮肤损伤 细胞凋亡 凋亡基因 创面愈合 

分 类 号：R818.74[医药卫生—放射医学]