一株绿僵菌的鉴定及其生物学特性  

Identification and Biological Characteristics of A Metarhizium pingshaense Strain Isolated from Melanotus cribricollis Larva

作　　者：张亚波[1] 吴盼盼[1] 王鹏[1] 刘剑[1] 徐天森[1] 王浩杰[1] 舒金平[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,富阳311400

出　　处：《林业科学》2012年第12期134-140,共7页Scientia Silvae Sinicae

基　　金：浙江省科技计划项目(2010C32036);国家林业局"948"项目(2007-4-22)

摘　　要：筛胸梳爪叩甲（Melanotus cribricollis）是浙江笋用林内昆虫的优势种群,其幼虫（即金针虫）是笋期主要害虫之一,在湖州市的德清和安吉、杭州市的余杭和临安等重要竹区的早园竹（Phyllostachys praecox）林中,鲜笋带虫率可达62%,种笋受害率更是高达80%以上,严重制约了当地竹产业的健康发展（徐天森,2004）。由于金针虫营地下生活,防治一直是竹笋生产管理的难点。An entomopathogenic fungal strain isolated from infected Melanotus cribricollis larvae was identified as Metarhizium pingshaense by both morphological and molecular methods.The 5＇ end of EF-1α of the isolate was sequenced,and submitted to GenBank with accession number JQ081264.Study on biological characteristics of the isolate showed that the optimum culture medium was PPDA.The range of optimum temperature for mycelium growth and conidia germination was from 25 ℃ to 30 ℃,respectively and the optimum temperature for conidia production was 25 ℃.The most suitable pH for conidia germination was 7.0.Conidia germination of this isolate could be obviously inhibited by ultraviolet radiation for less than 30 minutes.

关 键 词：平沙绿僵菌 EF-1α 筛胸梳爪叩甲 生物防治 

分 类 号：S718.7[农业科学—林学]