小麦^(60)Co诱变后代品质性状变异及高分子量谷蛋白亚基突变体鉴定  

Variation of Quality Characters in Wheat Radiated by ^(60)Co-ray and Identification of HMW-GS Mutants

作　　者：来德娥[1] 王敏[1] 张从宇[2] 赵平[1] 王志学[1] 

机构地区：[1]安徽农业大学农学院安徽作物生物学省级实验室,安徽合肥230036 [2]安徽科技学院,安徽凤阳233100

出　　处：《激光生物学报》2012年第6期563-568,共6页Acta Laser Biology Sinica

基　　金：国家科技支撑计划课题(2006BAD01A02);安徽作物生物学省级实验室项目(10111406024)

摘　　要：用300 Gy的60Co-γ射线辐射小麦品种涡9722的干种子,对M4代341个株系的蛋白质含量、湿面筋含量、沉降值、硬度进行变异分析,以超过群体均值±2×标准差为标准筛选出8个蛋白质与湿面筋含量较高的株系和4个蛋白质与湿面筋含量较低的株系。在M5代,进一步对这12个株系的品质性状、粉质参数和高分子量麦谷蛋白亚基(HMW-GS)进行了分析。结果表明,M5代部分变异株系的品质性状与亲本差异显著,粉质参数变异较大,11个变异株系与亲本具有明显不同HMW-GS谱带。亲本的HMW-GS组成为5+14+15+9+12,而变异株系的HMW-GS为1+5+7+9+12亚基或1+5+7+8+12亚基,说明这些株系的麦谷蛋白位点发生了变异。Dry seeds of wheat GUO 9722 were radiated by ^60Co-γ-ray with the dose of 300 Gy. Variations of protein content, gluten content, zeleny and hardness of 341 lines in M4 population were analyzed, and 12 variant lines including the 8 with higher protein and gluten contents and the 4 with the lower were selected based on a criterion out of the range of mean ± 2s. Their quality traits, farinogram parameters and high molecular weight glutenins （HMW-GS） were further an- alyzed in Ms generation. The result showed that there were significant differences in some quality traits, larger variations in farinogram parameters and obvious differences in electropheoretic patterns of HMW-GS between some variant lines and the parent. The HMW-GS of the parent was 5 ＋ 14 ＋ 15 ＋9 ＋ 12,while that of the variant lines was either 1 ＋5 ＋7 ＋9 ＋ 12 or 1 ＋ 5 ＋ 7 ＋ 8 ＋ 12, suggesting that variations of glutenin locus can be occurred.

关 键 词：小麦 60Co-γ射线 品质性状 粉质参数 HMW-GS 

分 类 号：S512.1[农业科学—作物学]