长爪沙鼠高脂血症造模方法的比较  

Comparison of the Methods of Developing Hyperlipidemia in Mongolian gerbils

作　　者：韦永芳[1] 钟欢妹 刘寒英[1] 梁成结[1] 

机构地区：[1]广州医学院实验动物中心,广州510182 [2]广州增城市人民医院检验科,广州511300

出　　处：《实验动物科学》2012年第6期22-25,29,共5页Laboratory Animal Science

基　　金：广东省科技计划项目(No.2010B060500017)

摘　　要：目的比较长爪沙鼠高脂血症模型的4种造模方法。方法选取用于复制大鼠高脂血症动物模型的4种配方饲料,饲喂长爪沙鼠60 d,分别在0 d、30 d、60 d空腹12 h后眼眶取血,测定血清中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)的含量,观察肝组织的形态学变化。结果4种高脂配方饲料在30 d、60 d均使长爪沙鼠血清的TC、HDL-C、LDL-C的值明显高于对照组,以TC升高最为明显,并且LDL-C的增加幅度明显大于HDL-C的增加,并出现不同程度的脂肪肝。结论 4种高脂配方饲料均可复制长爪沙鼠高脂血症模型。Objective To Compare four methods of developing hyperlipidemia in Mongolian gerbils. Method The gerbils in the experimental groups were fed respectively with four high-fat fodders with varying amounts of cholesterol, lard and cholate. The gerbils in the control group were fed with normal fodder. Serum levels of triglyceride （ TG ） , total cholesterol （ TC ） , low-density lipoprotein cholesterol （ LDL-C ） and high-density lipoprotein cholesterol （HDL-C） in the gerbils in all the groups were measured at the 0th, 30th, 60th day. Animals were sacrificed and the morphological changes of the liver were examined by histopathology. Result Serum levels of TC, LDL-C and HDL-C in gerbils of the experimental group were significantly higher than those in gerbils of the control group during the 30th,60th days, with a remarkable increase in serum TC level in particular, and increase of serum LDL-C were obviously bigger than the increase of serum HDL-C. There have been varying degrees of fatty liver in the experimental groups. Conclusion Four high-fat fodders can all cause hyperlipidemia in the gerbils.

关 键 词：长爪沙鼠 高脂血症 脂肪肝 

分 类 号：Q95-33[生物学—动物学]