三维多肽支架中前成骨细胞MC3T3-E1成骨分化的初步研究  

Osteogenic Differentiation of Preosteoblast Cells MC3T3-E1 in Three-dimensional Peptide Scaffolds

作　　者：陈婷[1] 卢婷利[1] 马玉樊[1] 王韵晴[1] 校月红[1] 李昱辉[1] 

机构地区：[1]西北工业大学生命学院、空间生物实验模拟技术国防重点学科实验室,西安710072

出　　处：《材料导报》2013年第2期48-51,共4页Materials Reports

基　　金：西北工业大学研究生创业种子基金(Z2011026)

摘　　要：在多肽EAK16水凝胶支架上接种小鼠前成骨细胞MC3T3-E1,采用倒置显微镜观察细胞形态,CCK-8(细胞计数试剂盒)检测细胞增殖情况。细胞在诱导培养基中培养1周后,观察不同时间段细胞碱性磷酸酶的分泌活性。采用ALP染色和茜素红-S染色作为定性实验研究MC3T3-E1向成骨方向的分化情况。结果表明,MC3T3-E1细胞在水凝胶支架EAK16上有较好的黏附和增殖能力,诱导培养后细胞有较高水平的碱性磷酸酶表达和矿化基质沉积。多肽水凝胶支架对前成骨细胞MC3T3-E1具有较好的生物相容性。Mouse preosteoblast MC3T3-E1 ceils were seeded into EAK16 hydrogel, the morphology was visualized under inverted phase contrast microscope, the cell growth was detected by CCK-8. Further cultured in osteogenic induction medium for a week, alkaline phosphatase （ALP） activities were evaluated by ELISA in different time period. As qualitation of osteoblast differentiation, ALP staining and Alizarin red-S staining were assessed. MC3T3- E1 cells show well adhesion and proliferation in the peptide hydrogel, high alkaline phosphatase activity and calcium deposits were detected after osteogenic induction. Peptide hydrogel scaffold exhibit excellent biocompatility to mouse preosteoblast MC3T3-E1 cells.

关 键 词：自组装多肽水凝胶 MC3T3-E1细胞 成骨分化 组织工程 

分 类 号：Q254[生物学—细胞生物学]