盐酸益母草碱对药流后大鼠内皮素介导的信号通路的影响  

Effect of leonurine hydrochloride on ET-mediated signal pathway in incomplete abortion in rats

作　　者：赵懿清[1] 李霞[1] 袁凤来[1] 贺春晖[1] 吕卫群[1] 

机构地区：[1]南通大学第三附属医院药剂科,江苏无锡214041

出　　处：《中国临床药理学杂志》2013年第1期59-61,67,共4页The Chinese Journal of Clinical Pharmacology

基　　金：江苏省中医药管理局中医药科技基金资助项目(LB09094);青年科学基金资助项目(81101372)

摘　　要：目的研究盐酸益母草碱对药流后大鼠内皮素(ET)及其受体介导的信号通路的影响。方法早孕大鼠灌服米非司酮和米索前列醇,制备不完全流产模型,连续给药7d后,取左侧子宫一半,用于ET和NO的测定;剩余部分冻存待测ET受体。培养右侧子宫平滑肌细胞,用于PLC的活性、[Ca2+]i和PKC的检测。结果盐酸益母草碱能明显提高血清ET水平及ET/NO比值,上调ETAmRNA及PKC蛋白的表达,增强PLC的活性;但对ETBmRNA的表达没有明显影响。结论盐酸益母草碱能通过调节ET介导的信号通路来促进药流后大鼠子宫的收缩,从而抑制其异常出血。Objective To explore the mechanism of leonurine hydro- chloride （LH） on uterine contractions in abortion rats. Methods Early pregnancies in rats had incomplete abortions induced using mifepristone in combination with misoprostol. After the abortions, the rats were treated with LH orally for 7 days and surgery was performed. The sinistro - uterus was dissected for measurement of endothelin（ET） and nitric oxide （NO）. The dextro - uterus was stored at - 80 ℃ for ETA and ETn analysis. Myometrial cells from the dextro - uterus were cultured for measurement of phospholipase C （PLC） activity, intra - cellular Ca2 ＋ concentra- tion （ [ Ca2 ＋ ] i）, and protein kinase C （PKC） activity. Results In vivo experiments, the ET level and ET/NO ratio were increased after LH treatment in rats with induced abortions and LH -can up -regulate ETA mRNA expression （P 〈0.01, vs the model group）, but there was not ETB mRNA change. LH significantly increased the [ Ca2 ＋ ] i, PLC activity, and relative production of PKC protein in myometrial cells. Conclusion LH increased uterine contractions in rats with incomplete abortions by modulating the ET receptor- mediated signal pathway.

关 键 词：盐酸益母草碱 内皮素 一氧化氮 胞内钙离子浓度 

分 类 号：R969.1[医药卫生—药理学]