蛋白质对纳米铕岛膜表面荧光的增强作用  

Surface Fluorescence Enhancement of Proteins on Nano-Europium Island Films

作　　者：李蔚[1] 陈金东[1] 焦燕妮[1] 周景洋[1] 刘海杰[2] 尹志娟[2] 国凯[2] 高希宝[2] 

机构地区：[1]山东省疾病预防控制中心,济南市经十路16992号250014 [2]山东大学公共卫生学院,济南市文化西路44号250012

出　　处：《光谱实验室》2013年第1期392-395,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：山东省科技发展计划项目(023100101)

摘　　要：将经硫辛酸修饰的铕纳米颗粒和蛋白质固定在石英玻璃表面或胶原蛋白隔离的石英玻璃表面,研究蛋白质对纳米铕岛膜荧光的增强作用。研究结果发现,在275nm激发波长下,铕纳米岛膜的荧光光谱与铕纳米颗粒溶液的荧光性质相似,且微量蛋白质的加入可以使铕纳米岛膜的荧光强度增强,但被石英玻璃片吸附后,铕纳米岛膜以及铕-蛋白质体系的荧光发射峰的位置由378.8nm红移至420nm,且胶原蛋白隔离铕纳米岛膜和滴加微量BSA蛋白质的荧光光谱相似,但荧光强度没有发生明显变化。Europium nanoparticles and the proteins were fixed on the surface of quartz slides or collagen quartz slides,the fluorescence enhancement of nano-metal island film by the proteins were studied.Results showed that under the excitation of 275nm,the fluorescent property of lipoic acid modified nano-europium island film are just familiar with that in solution,trace proteins can enhance the fluorescence intensity of europium nanoparticle system,and the emission peaks of nano-europium island film and protein-europium nano-island film are red shifted from 378.8nm in solution to 420nm. The fluorescent spectra of both nano-europium island film with the collagen and BSA on nanoeuropium island film with collagen are just the same,but the fluorescent intensities of BSA and nanoeuropium island film have no change.

关 键 词：表面荧光增强 纳米铕岛膜 蛋白质 

分 类 号：O657.32[理学—分析化学]