川芎嗪保护脓毒症大鼠心肌的抗氧化机制研究  

A research of protective effect of ligustrazine on myocardium in septic rats and its mechanism of anti- oxidation

作　　者：杨广[1] 黄丹虹[1] 杨澄[1] 李健[1] 张敏州[1] 

机构地区：[1]广东省中医院重症医学科,广东广州510120

出　　处：《中国中西医结合急救杂志》2013年第1期13-15,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：广东省自然科学基金（$2012010010397）;广州中医药大学科技创新基金项目（10CX048）

摘　　要：目的探讨川芎嗪对脓毒症大鼠心肌的保护作用及其抗氧化机制。方法将90只大鼠按随机数字表法分为假手术组（n=18）、模型组（n=36）、川芎嗪组（n=36），每组又分为24、48、72h3个时间点亚组，模型组与川芎嗪组每个时间点12只大鼠，假手术组6只大鼠。采用盲肠结扎穿孔术（CLP）复制脓毒症大鼠模型。川芎嗪组于制模后皮下注射川芎嗪注射液10mg／kg，假手术组和模型组给予等量生理盐水，均每日1次。于术后24、48及72h取大鼠腹主动脉血，测定血清心肌肌钙蛋白I（cTnI），取心肌组织测定超氧化物歧化酶（SOD）及丙二醛（MDA）水平。结果模型组及川芎嗪组术后各时间点cTnI（ng／L）、MDA（tamol／L）水平均较假手术组同期明显升高，于24h达峰值，48h后开始回落，而川芎嗪组升高程度低于模型组，24h、48h两组比较差异有统计学意义（24hcTnI：401．82±31．67比482．17±37．91，MDA：13．76±2．18比16．52±2．19；48hcTnI：353．57±38．19比415．77±28．64．MDA：11．05±1．94比14．98±2．43，均P〈0．05）；SOD活性（kU／L）较假手术组同期明显下降，于24h达谷值，48h后开始回升，而川芎嗪组降低程度明显小于模型组，两组比较差异有统计学意义（24h：128．54±5．50比117．83±6．61，48h：134．42±4．98比125．86±5．49，72h：139．49±5．74比131．57±4．98，均P〈0．05）。结论川芎嗪可能通过清除氧自由基、抗氧化机制实现改善脓毒症时心肌损伤的作用。Objective To investigate the protective effect of ligustrazine on myocardium in septic rats and its possible mechanism of anti-oxidation. Methods Ninety male rats were randomly divided into sham operated group （n= 18）, model group （n=36）, and ligustrazine treated group （n=36）. Each group was subdivided into three subgroups according to the time point of sacrifice ： 24,48 and 72 hours. There were 12 rats in model or ligustrazine subgroup at each time point, while in sham operated group, 6 rats in each subgroup. The rat models were subjected to sepsis induced by cecal ligation and puncture （CLP）. Rats in ligustrazine group were subcutaneously injected with ligustrazine injection 10 mg/kg, and those in model group and sham operated group were injected the same dosage of normal saline, once a day after modeling. The abdominal aorta blood of rats was taken out at 24,48 and 72 hours after operation, the levels of serum cardiac troponin I （cTnI） were measured, and the contents of superoxide dismutase （SOD） and malonaldehyde （MDA） in myocardium were detected at the same time points. Results Compared with sham operated group, the levels of cTnI （ng/L） and MDA （p^mol/L） were increased significantly in rats of model group and ligustrazine group at each time point, they peaked at 24 hours and decreased after 48 hours. The degree of elevation of ligustrazine group was lower than that in model group, and there were statistical significant differences between the two groups （24 hours cTnI ：401.82＋31.67 vs. 482.17＋37.91, MDA ： 13.76＋2.18 vs. 16.52＋2.19 ; 48 hours cTnI ： 353.57＋38.19 vs. 415.77＋28.64, MDA ： 11.05＋ 1.94 vs. 14.98＋2.43, all P〈0.05）. Compared with sham operated group, the activities of SOD （kU/L） were decreased significantly in rats of model group and ligustrazine group at the same time points, they reached their valley values at 24 hours and began to ascend after 48 hours. The degree of reduction of ligustrazine group was lower than that in mod

关 键 词：脓毒症 心肌保护 川芎嗪 

分 类 号：R277.733.3[医药卫生—中医学]