青蒿素对脑缺血／再灌注损伤大鼠肿瘤坏死因子-α表达的影响  

The effect of Artemisia annua on expression of tumor necrosis factor- a in rats with acute cerebral ischemia/ reperfusion injury

作　　者：罗悦晨[1] 梁林[2] 臧照辉[3] 董化江[4] 杨德慧[4] 李伯森[4] 单云官[4] 

机构地区：[1]天津医科大学研究生院.天津300070 [2]武警后勤学院科研部 [3]武警后勤学院研究生处 [4]武警后勤学院人体解剖学教研室,民政部101研究所遗体防腐整容重点实验室,天津300162

出　　处：《中国中西医结合急救杂志》2013年第1期27-29,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：“十一五”国家科技支撑计划（2007BAK38805）;武警医学院种子基金项目（FYM201210）

摘　　要：目的探讨青蒿素对脑缺血／再灌注（I／R）损伤大鼠肿瘤坏死因子-α（TNF-α）的影响。方法将40只Wistar大鼠按随机数字表法分为假手术组、模型组及青蒿素低、中、高浓度组，每组8只。采用大脑中动脉闭塞法（MCAO）复制局灶性脑I／R损伤大鼠模型。制模成功后，假手术组和模型组均灌胃30ml生理盐水，青蒿素低、中、高浓度组分别由腹腔注射青蒿素200、300、400mg／kg，均每日1次，连续60d。采用放射免疫法（RIA）检测血清TNF—α含量，逆转录-聚合酶链反应（RT—PCR）和蛋白质免疫印迹法（Westernblotting）检测关节软骨组织TNF—O／．mRNA和蛋白表达。结果与假手术组比较，模型组和青蒿素各浓度组血清TNF—α含量和组织TNF—α．mRNA及蛋白表达均明显升高；与模型组比较，青蓠素各浓度组血清TNF—α含量及组织TNF—α．mRNA和蛋白表达均明显下降（均P〈0．05），以青蒿素高浓度组下降更显著[血清TNF-α（μg／L）：51．9±9．4比88．3±9．7，TNF—dmRNA：0．20±0．14比0．77±0.31，TNF—α蛋白：0．19±0．08比0．76±0．09，均P〈0．05]。结论青蒿素可降低脑I／R损伤大鼠TNF—d的表达，有效控制炎症反应进程。Objective To detect the effect of Artemisia annua on tumor necrosis factor-ct （TNF-α ） in rats with acute cerebral ischemia/reperfusion （I/R） injury. Methods Forty Wistar rats were randomized into sham operation group, model group, Artemisia annua high-, middle- and low-dose groups, each n= 8. The model of focal cerebral I/R injury was reproduced by middle cerebral artery occlusion （MCAO） . In the sham operation group and model group, 30 ml normal saline was administered intra-gastrically, while in Artemisia annua low-, middle- and high-dose groups,200,300 and 400 mg/kg Artemisia annua were intra-peritoneally injected, respectively, once a day for consecutive 60 days in all the groups. The serum content of TNF- α was detected by radioimmunoassay, and reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting methods were used to determine the expressions of tissue TNF- α mRNA and protein. Results Compared with the sham operation group, the concentration of TNF- α in serum, and the expressions of tissue TNF- α mRNA and protein in model group and various Artemisia annua groups were significantly increased ; compared with the model group, the concentration of TNF- α in serum, and the expressions of tissue TNF-α mRNA and protein in various Artemisia annua groups were significantly decreased （all P〈 0.05）, the greatest descent being in the high-dose group [ TNF- ct in serum （p.g/L） ： 51.9＋9.4 vs. 88.3＋9.7, TNF-α mRNA ： 0.20＋0.14 vs. 0.77＋0.31, TNF- α protein ： 0.19＋0.08 vs. 0.76＋0.09, all P〈 0.051. Conclusion Artemisia annua can down-regulate the expression of TNF- α and effectively inhibit the process of inflammation in rates with acute cerebral I/R injury.

关 键 词：青蒿 缺血 再灌注损伤 脑 肿瘤坏死因子-Α 大鼠 

分 类 号：R255.1[医药卫生—中医内科学]