孕妇血清中早孕因子的纯化及其部分特性研究  

ISOLATION AND PARTIAL CHAPACTERIZATION OF EARLY PREGNANCY FACTOR (EPF) FROM HUMAN PREGNANCY SERUM

作　　者：徐振山[1] 苏宝田[1] 史敏言[1] 谭福莱 

机构地区：[1]安徽医科大学病理生理学教研室 [2]安徽省医学科学研究所

出　　处：《蚌埠医学院学报》1991年第3期164-167,共4页Journal of Bengbu Medical College

摘　　要：采用DEAE纤维素(Whatman产)柱层析Sephadex G-100凝胶过滤、聚丙烯酰胺凝胶圆盘电泳(Disc-PAGE)、SDS-聚丙烯酰胺凝胶平板电泳(SDS-PAGE)对50例妊娠3～8周妇女混合血清中早孕因子(EPF)进行纯化提取及部分特性研究,并采用花结抑制试验(Ea-RIT)检测EPF活性,结果表明:(1)EPF存在于早孕妇女血清中;(2)早孕血清中EPF的活性形式主要为21.2KD、26.3KD的蛋白多肽;(3)EPF有明显的免疫抑制活性。This paper describes the isolation and partial characterization of EPF in the mixed sera of SO pregnancy women , between 3 and 8 weeks of gestation, EPF was purified with use of Ion - Exchange Chromatography, Sephades G-100 Gel Permeation , Disc-PAGE and SDS-PAGE. EPF activity was measured in all the serum samples and chromatogiaphic fractions using the rosette inhilition test. The results showed: 1. EPF is present in maternal scrum during carly hu-man gestation. 2. Active forms of Fuman EPF is mainly polypeptide of 21.2 KD and 26.3 KD. 3.EPF has distinct properties of immu-no-supprcssion.

关 键 词：早孕因子 花结抑制试验 孕妇 

分 类 号：R446.63[医药卫生—诊断学]