大肠杆菌的产毒能力与血清型关系——Ⅰ 被动免疫溶血试验(PIH)检测产肠毒素大肠杆菌不耐热肠毒素  

DETECTION OF HEAT LABILE ENTEROTOXIN OF E. COLI WITH PASSIVE IMMUNE HEMOLYSIS TECHNIQUE

作　　者：王克智[1] 陈艺林[1] 汤秀兰[1] 王群[1] 

机构地区：[1]蚌埠医学院检验系临床微生物学免疫学教研室

出　　处：《蚌埠医学院学报》1991年第3期161-163,共3页Journal of Bengbu Medical College

基　　金：省教委资助项目

摘　　要：本文自腹泻患儿粪便中分离155株大肠杆菌,经用PIH和Biken's两种方法对产肠毒素(LT)大肠杆菌的检测,检出率分别为8.3％和6.4％,远低于国外有关报道。实验用9株LT^+大肠杆菌的参考菌株经两种方法检测全部阳性。15株非产肠毒素肠道细菌,LT毒素检测均为阴性。PIH方法检测LT^+ETEC,在特异性和敏感性方面,均相当于放射免疫和基因探针技术,但更快速简便。This paper reports the detection of 155 strains E. coli from children with acute diarrhea. PIH assay was compared with Biken's test to identify LT+ETEC. The results obtained were 8.3% and 6.4% respectively, which were much lower than those reported abroad. The 9 reference strains of LT+ETEC detected by both methods were all positive, whereas the 15 strains of non-enterotoxin producing en-teropathogens were negative. The PIH assay showed specificity and sensitivity by comparison with gene hybridizable and RIA technique, and presented to be more was rapid and simpler in clinical practice.

关 键 词：大肠杆菌 肠毒素 免疫学 

分 类 号：R378.21[医药卫生—病原生物学]