男性不育症患者精子DNA完整性与精液分析参数相关性研究  

Correlation between sperm DNA integrity and semen parameters in male infertility

作　　者：龚道元[1] 李子萍[1] 庄锡伟 郝兴霞[3] 康梅[1] 黄勇汉[4] 

机构地区：[1]佛山科学技术学院医学院,广东佛山528000 [2]佛山市禅城区中心医院,广东佛山528000 [3]内蒙古医学院基础医学院,内蒙古呼和浩特010059 [4]佛山市第一人民医院,广东佛山528000

出　　处：《国际检验医学杂志》2012年第19期2321-2322,2324,共3页International Journal of Laboratory Medicine

基　　金：广东省自然科学基金资助项目(9152902001000017)

摘　　要：目的探讨男性不育症患者精子DNA完整性与精液参数间的相关性。方法已有生育男性健康者50例(对照组);男性不育症患者100例(不育组),根据精子常规参数检测结果分为参数正常不育组及参数异常不育组。采用计算机辅助精液分析系统分析精液常规和运动参数,采用末端脱氧核苷酸转移酶介导的dUTP末端标记法检测精子DNA碎片化指数(DFI)。结果参数正常不育组和参数异常男性不育组精子DFI均高于对照组(P<0.05),参数异常不育组精子DFI高于参数正常不育组(P<0.05);不育组精子DFI与精子密度、正常形态率、活动率、存活率、a级和b级精子活力及精子运动参数(直线速度、曲线速度、平均路径速度和侧摆幅度)呈不同程度负相关(P<0.05),与c级和d级精子活力呈正相关(P<0.05),与精子直线性、前向性、摆动性和鞭打频率无相关性(P>0.05)。结论精子DFI是可用于评估精液质量和男性生育能力的理想指标。Objective To study the correlation between sperm DNA infertility and semen parameters of male infertility.Methods 50 healthy fertile male were enrolled as control group.100 male with infertility were enrolled as infertility group,and were divided into parameter-normal and parameter-abnormal infertility group.Computer aided semen analysis was performed for the detection of routine and movement parameters,terminal deoxynucleotidyl transferase mediated-dUTP nick end labeling assay was used for the detection of DNA fragmentation index（DFI）.Results There was significant difference of DFI between control group,parameter-normal group and parameter-abnormal group（P0.05）.DFI in infertility group was negatively correlated with sperm density,sperm morphology,activity rate,survival rate,a and b class of sperm motility and sperm motion parameters of linear velocity,curvilinear velocity,average path velocity and sway amplitude with different extent（P0.05）,positively correlated with c and d class of sperm motility（P0.05）,and was not correlated with sperm line,forward,swinging and beat frequency（P0.05）.Conclusion Sperm DFI could be a better evaluation parameter of semen quality and male fertility.

关 键 词：不育 男性 精子 DNA 精液分析 

分 类 号：R698.2[医药卫生—泌尿科学]