溶血、脂血、黄疸对同型半胱氨酸测定的影响  

Influence of Hemolysis,Lipaemia or Icterus on the Results of Homocysteine Assay

作　　者：张巧云[1] 龙宪连[1] 欧红玲[1] 贾连玲[1] 王欣茹[1] 

机构地区：[1]第二炮兵总医院检验科,北京100088

出　　处：《国际检验医学杂志》2012年第20期2460-2462,共3页International Journal of Laboratory Medicine

摘　　要：目的观察溶血、脂血、黄疸对同型半胱氨酸(homocysteine,Hcy)测定的影响。方法 10例正常血清用血红蛋白液配制成溶血程度为5、10、15、20、25、30g/L的系列血清,10例正常血清用脂肪乳配制成三酰甘油(TG)为2、4、8、16mmol/L的系列血清,10例正常血清用胆红素标准品配制成总胆红素(TBIL)为20、40、80、160μmol/L的系列血清,采用全自动生化分析仪检测上述30例标本Hcy的变化,并与正常血清Hcy水平进行比较。结果溶血程度为5、10、15、20、25、30g/L时,Hcy降低程度依次为0.9%(0.2%～3.4%)、2.3%(0.4%～12.2%)、8.6%(1.8%～29.4%)、66.2%(47.8%～90.0%)、88.0%(82.2%～96.7%)、102.9%(97.7%～116.3%)(此时大多数Hcy检测值为负值)。不同浓度TG和TBIL下的Hcy检测值差异无统计学意义(P>0.05)。结论溶血对Hcy的测定有显著影响,脂血、黄疸对Hcy的测定无明显影响。Objective To observe the influence of hemolysis,lipaemia or icterus on the results of homocysteine assay. Methods Ten normal sera samples were prepared into series with 5,10,15,20,25,30 g/L of hemolysis degree by adding hemoglobin solu lion ; ten normal sera samples were prepared into series with 2,4,8,16 mmol/L triglyceride（TG） by adding fat emulsion; ten normal sera samples were prepared into series wilh 20,40,80,160 1,mol/L total bilirubin（TBIL） by adding bilirubin standard preparalhm= and Hey levels were assayed in these samples with automatic biochemistry analyzer. Results When hemolysis degree was 5, 1 0,15. 20,25,30g/L,respectivelythe accordingly decreased extent of Hcy was 0. 9%（0. 2%--3.4%）,2. 3% （0. 4%- 12. 2%）. 8. 6%（1.89%—29.4%）.64.%（47. 8- 90.0%）,88.0%（82.2%-96.7% ）,102.9%（97.7%--116.3%）（when the value of Hey lest in most samples was negative）, However,Hcy values showed no significant difference in samples with higher concentration of TG and TBII.（ P〉0.05）. Conclusion Hey assay were influenced by hemolysis significantly,but not by lipaemia or icterus.

关 键 词：同型半 忧氯酸 溶血 脂血 黄疸 

分 类 号：R446.1[医药卫生—诊断学]