EDTA诱导假性血小板减少症的临床检验诊断  

Clinical laboratory diagnostics of ethylenediaminetetraacetic acid-dependent pseudothrombocytopenia

作　　者：冯戟[1] 罗丹[1] 王照峰[1] 赵瑞敏[1] 刘晓莉[1] 

机构地区：[1]空军总医院临床检验中心,北京100142

出　　处：《国际检验医学杂志》2012年第21期2592-2593,共2页International Journal of Laboratory Medicine

摘　　要：目的通过研究EDTA依赖性假性血小板减少症(EDTA-PTCP)患者的临床实验室特征以避免临床的误诊。方法采集EDTA-PTCP患者EDTA与枸橼酸钠抗凝的静脉血,在全血细胞分析仪上检测这些标本的PLT,另外将EDTA与枸橼酸钠抗凝血制成的血涂片用于显微镜检。采集所有患者的指血用于手工PLT计数。结果 EDTA抗凝法计数PLT与枸橼酸钠抗凝法及手工法进行比较差异具有统计学意义(P<0.05)。EDTA抗凝法计数PLT明显低于枸橼酸钠抗凝法及手工法(P<0.05)。枸橼酸钠抗凝法与手工法计数PLT相比差异无统计学意义(P>0.05)。结论 EDTA可导致PLT假性减少。当PLT减少时,应采用枸橼酸钠抗凝血检测及人工计数PLT进行确认,以避免EDTA-PTCP的误诊与误治。Objective To investigate the clinical laboratory features of patients with ethyienediaminetetraacetic acid-dependent pseudothrombocytopenia（EDTA PTCP） to avoid incorrect diagnosis. Methods Venous blood samples of patients with EDTA- PTCP were collected with dipotassium EDTA and sodium citrate respectively. PLT counting of which were measured on hematology analyzer,and blood smears of which were made for microscope observation. Finger blood samples were also collected, which were used for manual PI.F counting. Results There were significant difference of platelets counting among EDTA and citrate anticoagu- lants and manual methods （P〈0. 05）. For EDTA-K2 anticoagulated blood, PLT is significantly lower than citrate anticoagulated blood and finger blood and there were no significant difference between citrate anticoagulated-blood and finger blood（P〉0.05）. Conclusion EDTA-K2 can accelerate the PLT aggregation and cause PTCP. When platelet reduction is discovered,sodium citrate anticoagulant blood sample and peripheral blood manual PLT counting should be taken to verif PLT counting and to avoid patients with EDTA-PTCP misdiagnosis and mistreatment.

关 键 词：血小板减少 依地酸 诊断 

分 类 号：R446.1[医药卫生—诊断学]