鲍曼不动杆菌耐药性与分子分型相关性研究  

Preliminary study of the relationship between drug sensitivity and molecular typing of of acinetobacter baumannii

作　　者：尤涛[1] 辛那 井发红 康炜[1] 

机构地区：[1]西安医学院附属医院,陕西西安710077

出　　处：《国际检验医学杂志》2012年第22期2717-2718,共2页International Journal of Laboratory Medicine

摘　　要：目的初步研究11株鲍曼不动杆菌临床分离株药敏试验结果与脉冲场电泳分子分型结果之间的相关性。方法利用常规方法对从临床标本中分离的鲍曼不动杆菌进行培养鉴定后进行药敏实验,利用脉冲场电泳方法进行分子分型,并比较二者结果。结果 4株对所有抗菌药物均敏感的菌株带型数为10～13条,而1株对所有抗菌药物全耐药为19条带型,其余6株带型在二者之间。结论分子分型结果初步可以显示耐药情况,相同的分子分型菌株药敏试验结果一致性较高。Objective To study the correlation between the antimicrobial susceptibility and pulsed-held gel electrophoresls mo- lecular classification of 11 strains of acinetobacter baumannii. Methods 11 strains of acinetobacter baumannii were isolated from clinical samples. Conventional methods were used for culturing and drug sensitivity testing. Pulsed-field gel electrophoresis method was performed for molecular typing. The two results were compared by using statistical methods. Results 4 strains that sensitive to all antibiotics had 10-13 electrophoresis strips,and 1 strain that resistance to all antibiotics had 19 electrophoresis strips. The rest strains were with electrophoresis strip between the two results mentioned above. Conclusion Molecular typing results could pre- liminaryly show drug resistance,and molecular typing of the same results,higher consistency of antibiotic susceptibility.

关 键 词：鲍氏不动杆菌 微生物敏感性试验 电泳 细菌分型技术 

分 类 号：R446.5[医药卫生—诊断学]