Bone marrow-derived mesenchymal stem cells increase dopamine synthesis in the injured striatum  

作　　者：Yue Huang Cheng Chang Jiewen Zhang Xiaoqun Gao 

机构地区：[1]Department of Neurology, Henan Provincial People's Hospital [2]Department of Anatomy, Zhengzhou University

出　　处：《Neural Regeneration Research》2012年第34期2653-2662,共10页中国神经再生研究（英文版）

基　　金：supported by grants from the Ministryof Health of China, No. 2011010009;the Science and Technology Department of Henan Province, No.112102310230

摘　　要：Previous studies showed that tyrosine hydroxylase or neurturin gene-modified cells transplanted into rats with Parkinson＇s disease significantly improved behavior and increased striatal dopamine content. In the present study, we transplanted tyrosine hydroxylase and neurturin gene-modified bone marrow-derived mesenchymal stem cells into the damaged striatum of Parkinson＇s disease model rats. Several weeks after cell transplantation, in addition to an improvement of motor function tyrosine hydroxylase and neurturin proteins were up-regulated in the injured striatum, and importantly, levels of dopamine and its metabolite 3,4-dihydroxyphenylacetic acid increased significantly. Furthermore, the density of the D2 dopamine receptor in the postsynaptic membranes of dopaminergic neurons was decreased. These results indicate that transplantation of tyrosine hydroxylase and neurturin gene-modified bone marrow-derived mesenchymal stem cells increases dopamine synthesis and significantly improves the behavior of rats with Parkinson＇s disease.Previous studies showed that tyrosine hydroxylase or neurturin gene-modified cells transplanted into rats with Parkinson＇s disease significantly improved behavior and increased striatal dopamine content. In the present study, we transplanted tyrosine hydroxylase and neurturin gene-modified bone marrow-derived mesenchymal stem cells into the damaged striatum of Parkinson＇s disease model rats. Several weeks after cell transplantation, in addition to an improvement of motor function tyrosine hydroxylase and neurturin proteins were up-regulated in the injured striatum, and importantly, levels of dopamine and its metabolite 3,4-dihydroxyphenylacetic acid increased significantly. Furthermore, the density of the D2 dopamine receptor in the postsynaptic membranes of dopaminergic neurons was decreased. These results indicate that transplantation of tyrosine hydroxylase and neurturin gene-modified bone marrow-derived mesenchymal stem cells increases dopamine synthesis and significantly improves the behavior of rats with Parkinson＇s disease.

关 键 词：Parkinson＇s disease tyrosine hydroxylase NEURTURIN bone marrow-derived mesenchymal stemcells transplantation dopamine gene therapy neurodegenerative disease regeneration neural 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]