Transactivating-transduction protein-polyethylene glycol modified liposomes traverse the blood-spinal cord and blood-brain barriers  

作　　者：Xianhu Zhou Chunyuan Wang Shiqing Feng Jin Chang Xiaohong Kong Yang Liu Shijie Gao 

机构地区：[1]Department of Orthopedic Surgery, Tianjin Medical University General Hospital [2]School of Material Science and Engineering, Tianjin University [3]Medicine College, Nankai University

出　　处：《Neural Regeneration Research》2012年第35期2784-2792,共9页中国神经再生研究（英文版）

基　　金：sponsored by grants from the National Natural Science Foundation of China,No.30872603;the New Century Excellent Talents Program of the Ministry of Education of China,No.NCET-06-0251;the Applied Basic Research Programs of Science and Technology Commission Foundation of Tianjin,China,No.07JCYBJC10200

摘　　要：Naive liposomes can cross the blood-brain barrier and blood-spinal cord barrier in small amounts. Liposomes modified by a transactivating-transduction protein can deliver antibiotics for the treatment of acute bacterial infection-induced brain inflammation. Liposomes conjugated with polyethylene glycol have the capability of long-term circulation. In this study we prepared transactivating-transduction protein-polyethylene glycol-modified liposomes labeled with fiuorescein isothiocyanate. Thus, liposomes were characterized by transmembrane, long-term circulation and fluorescence tracing. Uptake, cytotoxicity, and the ability of traversing blood-spinal cord and blood-brain barriers were observed following coculture with human breast adenocarcinoma cells （MCF-7）. Results demonstrated that the liposomes had good biocompatibility, and low cytotoxicity when cocultured with human breast adenocarcinoma cells. Liposomes could traverse cell membranes and entered the central nervous system and neurocytes through the blood-spinal cord and blood-brain barriers of rats via the systemic circulation. These results verified that fluorescein isothiocyanate-modified transactivating-transduction protein-polyethylene glycol liposomes have the ability to traverse the blood-spinal cord and blood-brain barriers.Naive liposomes can cross the blood-brain barrier and blood-spinal cord barrier in small amounts. Liposomes modified by a transactivating-transduction protein can deliver antibiotics for the treatment of acute bacterial infection-induced brain inflammation. Liposomes conjugated with polyethylene glycol have the capability of long-term circulation. In this study we prepared transactivating-transduction protein-polyethylene glycol-modified liposomes labeled with fiuorescein isothiocyanate. Thus, liposomes were characterized by transmembrane, long-term circulation and fluorescence tracing. Uptake, cytotoxicity, and the ability of traversing blood-spinal cord and blood-brain barriers were observed following coculture with human breast adenocarcinoma cells （MCF-7）. Results demonstrated that the liposomes had good biocompatibility, and low cytotoxicity when cocultured with human breast adenocarcinoma cells. Liposomes could traverse cell membranes and entered the central nervous system and neurocytes through the blood-spinal cord and blood-brain barriers of rats via the systemic circulation. These results verified that fluorescein isothiocyanate-modified transactivating-transduction protein-polyethylene glycol liposomes have the ability to traverse the blood-spinal cord and blood-brain barriers.

关 键 词：liposomes transactivating-transduction protein polyethylene glycol blood-spinal cord barrier blood-brain barrier caudal vein fiuorescein isothiocyanate rat human breast adenocarcinomacells （MCF-7） cytobiology neural regeneration 

分 类 号：R318.0[医药卫生—生物医学工程]