Inducible expression of endomorphins in murine dendritic cells  

作　　者：Xiaohuai Yang Hui Xia Yong Chen Xiaofen Liu Cheng Zhou Qin Gao Zhenghong Li 

机构地区：[1]Department of Urinary Surgery, First Hospital of Bengbu Medical College [2]Anhui Key Laboratory of Infection & Immunity, Bengbu Medical College [3]Department of Physiology, Bengbu Medical College

出　　处：《Neural Regeneration Research》2012年第35期2811-2817,共7页中国神经再生研究（英文版）

基　　金：supported by the Natural Science Research Foundation of Anhui Province Universities,No.KJ2011A202;the National Natural Science Foundation of China,No.81000074

摘　　要：Bone marrow precursor cells were extracted from C57BL/6J mice aged 7-8 weeks, and dendritic cells were purified using anti-CD1 lc （a specific marker for dendritic cells） antibody-coated magnetic beads. Immunofluorescence staining revealed that the expression levels of endomorphin-1 and endomorphin-2 were upregulated in dendritic cells activated by lipopolysaccharide. An enzyme Jmmunoassay showed that lipopolysaccharide and other Toll-like receptor ligands promoted the secretion of endomorphin-1 and endomorphin-2 from activated dendritic cells. [3H]-thymidine incorporation demonstrated that endomorphin-1 and endomorphin-2 both inhibited the proliferation of T lymphocyte induced by activated dendritic cells. Furthermore, this immunosuppressive effect was blocked by CTOP, a specific antagonist of IJ-opioid receptors. Our experimental findings indicate that activated dendritic cells can induce the expression and secretion of endomorphins, and that endomorphins suppress T lymphocyte proliferation through activation of iJ-opioid receptors.Bone marrow precursor cells were extracted from C57BL/6J mice aged 7-8 weeks, and dendritic cells were purified using anti-CD1 lc （a specific marker for dendritic cells） antibody-coated magnetic beads. Immunofluorescence staining revealed that the expression levels of endomorphin-1 and endomorphin-2 were upregulated in dendritic cells activated by lipopolysaccharide. An enzyme Jmmunoassay showed that lipopolysaccharide and other Toll-like receptor ligands promoted the secretion of endomorphin-1 and endomorphin-2 from activated dendritic cells. [3H]-thymidine incorporation demonstrated that endomorphin-1 and endomorphin-2 both inhibited the proliferation of T lymphocyte induced by activated dendritic cells. Furthermore, this immunosuppressive effect was blocked by CTOP, a specific antagonist of IJ-opioid receptors. Our experimental findings indicate that activated dendritic cells can induce the expression and secretion of endomorphins, and that endomorphins suppress T lymphocyte proliferation through activation of iJ-opioid receptors.

关 键 词：dendritic cells ENDOMORPHIN I J-receptor antigen-presenting cell nerve immunization neuralregeneration 

分 类 号：R392[医药卫生—免疫学]