Panax notoginseng saponin attenuates hypoxia/reoxygenation-induced oxidative stress in cortical neurons  

作　　者：Chen Yan Jinqiang Zhu Xiaoxu Jia Chao Wang Shaoxia Wang Liyuan Kang 

机构地区：[1]Key Laboratory for Prescription of National Educational Ministry of China,Research Center of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine

出　　处：《Neural Regeneration Research》2012年第36期2853-2859,共7页中国神经再生研究（英文版）

基　　金：supported by the National Basic Research Program of China (973 Program),No.2005CB523404;the National Natural Science Foundation, No.30901922;Science and Technology Development Fund forUniversities in Tianjin,No.2006305

摘　　要：The present study monitored the effect of 2, 10, and 50 mg/L of Panax notoginseng saponin exposure following hypoxia-reoxygenation injury in fetal rat cortical neurons. Results showed that varying doses of Panax notoginseng saponin significantly enhanced the cell viability of neurons, reduced malondialdehyde content, increased superoxide dismutase activity, inhibited mRNA and protein expression of inducible and neuronal nitric oxide synthase, and decreased the release of nitric oxide in hypoxia/reoxygenation injured cells. In particular, 50 mg/L of Panax notoginseng saponin was the most effective dose. These findings suggest that Panax notoginseng saponin can attenuate neuronal oxidative stress injury caused by hypoxia/reoxygenation in a dose-dependent manner.The present study monitored the effect of 2, 10, and 50 mg/L of Panax notoginseng saponin exposure following hypoxia-reoxygenation injury in fetal rat cortical neurons. Results showed that varying doses of Panax notoginseng saponin significantly enhanced the cell viability of neurons, reduced malondialdehyde content, increased superoxide dismutase activity, inhibited mRNA and protein expression of inducible and neuronal nitric oxide synthase, and decreased the release of nitric oxide in hypoxia/reoxygenation injured cells. In particular, 50 mg/L of Panax notoginseng saponin was the most effective dose. These findings suggest that Panax notoginseng saponin can attenuate neuronal oxidative stress injury caused by hypoxia/reoxygenation in a dose-dependent manner.

关 键 词：Panax notoginseng saponin neuron oxidative stress superoxide dismutase MALONDIALDEHYDE nitric oxide neuroprotection nerve injury neural regeneration 

分 类 号：R285[医药卫生—中药学]