辛伐他汀凝胶对Ⅱ度根分叉牙槽骨缺损再生的影响  

Effects of simvastatin gel on bone regeneration following grade Ⅱ furcation of alveolar defects

作　　者：陈珊[1] 杨军英[1] 张盛炎[1] 任静[1] 周倩冰[1] 

机构地区：[1]中山大学附属第一医院口腔科,广东省广州市510080

出　　处：《中国组织工程研究》2012年第51期9501-9506,共6页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技计划项目(2009B060700042);课题名称:辛伐他汀局部给药缓释剂型的研制及其对牙槽骨重建的作用~~

摘　　要：背景:研究表明他汀类药物具有刺激骨新生的作用。目的:观察辛伐他汀/甲基纤维素凝胶对小型猪根分叉区牙槽骨缺损后骨再生的影响。方法:制备小型猪上下颌两侧第三、四前磨牙Ⅱ度根分叉牙槽骨缺损模型,随机分为5组:低、中、高浓度辛伐他汀/甲基纤维素凝胶组于人工根分叉缺损处分别注射0.01,0.03,0.044g/mL辛伐他汀/甲基纤维素凝胶50μL,对照组于人工根分叉缺损处注射甲基纤维素凝胶50μL,1次/周,连续8周;空白对照组不作任何处理。结果与结论:与对照组和空白对照组比较,低、中、高浓度辛伐他汀/甲基纤维素凝胶组根分叉区牙槽骨高度明显修复(P<0.001),新生牙槽骨几乎充满根分叉区,可见骨小梁成网状结构及成熟的哈佛氏系统,其中低浓度辛伐他汀/甲基纤维素凝胶组新生骨成熟度较中、高浓度辛伐他汀/甲基纤维素凝胶组高,且炎症细胞浸润较少。对照组与空白对照组未见明显新生骨,仅见少量新生骨。表明局部应用辛伐他汀/甲基纤维素凝胶可刺激根分叉区牙槽骨缺损后的骨再生,适宜剂量是0.01g/mL。BACKGROUND：Studies have shown that statins can stimulate the bone regeneration. ONJECTIVE：To investigate the effects of simvastatin/methylcellulose gel on bone regeneration after grade Ⅱ furcation of alveolar defects in miniature pigs. METHODS：Grade Ⅱ furcation of alveolar defects model were produced in 32 teeth （the third and fourth premolars） of miniature pigs. The 32 experimental teeth were divided into five groups：the low-, middle-and high-dose simvastatin/methylcellulose gel groups were injected with 0.01, 0.03 and 0.044 g/mL simvastatin/methylcellulose gel 50 μL into the furcation sites; the control group was injected with 50 μL simvastatin/methylcellulose gel into the furcation sites once a week and lasted for 8 weeks; the blank control group had no treatment. RESULTS AND CONCLUSION：Compared with the control group and blank control group, the height of the alveolar bone in the furcation sites of low-, middle-and high-dose simvastatin/methylcellulose gel groups were increased （P 0.001）, and the furcation sites were filled with the new alveolar bone, the trabecular was in the network structure and the mature Harvard’s system. The maturity of the new alveolar bone in the low-dose simvastatin/methylcellulose gel group was higher than that in the middle-and high-dose simvastatin/methylcellulose gel groups with less inflammatory cell infiltration. Little new alveolar bone could be seen in the control group and blank control group. Local application of simvastatin/methylcellulose gel can stimulate the regeneration of alveolar bone in furcation defect sites and the best dose of simvastatin/methylcellulose gel is 0.01 g/mL.

关 键 词：辛伐他汀 甲基纤维素凝胶 Ⅱ度根分叉缺损 牙周骨组织再生 新生骨 小型猪 

分 类 号：R318[医药卫生—生物医学工程]