Pluronic F127/聚乳酸纳米粒子的药物作用  

Pluronic F127/poly(lactic acid) nanoparticles as drug carriers

作　　者：李资玲[1] 黄优生[2] 熊向源[1] 龚妍春[1] 李玉萍[1] 

机构地区：[1]江西科技师范大学生命科学学院,江西省南昌市330013 [2]江西省分析测试中心,江西省南昌市330029

出　　处：《中国组织工程研究》2012年第51期9529-9532,共4页Chinese Journal of Tissue Engineering Research

基　　金：江西省教育厅科学技术研究项目(GJJ10594)~~

摘　　要：背景:国内关于纳米粒子在水及磷酸盐缓冲液中稳定性的研究报道较少。目的:考察PluronicF127/聚乳酸纳米粒子在水及磷酸盐缓冲液中的稳定性及PluronicF127/聚乳酸聚合物作为药物载体的可行性。方法:采用透析法制备PluronicF127/聚乳酸纳米粒子,通过高效液相色谱研究该纳米粒子作为药物载体包埋紫杉醇及体外释放行为,同时采用MTT法考察该聚合物的细胞毒性。结果与结论:PluronicF127/聚乳酸纳米粒子在水中的稳定性优于磷酸盐缓冲液,其次,温度对于纳米粒子的稳定性影响较大,无论是磷酸盐缓冲液还是水中,37℃条件下粒径变化均较大,因此纳米粒子在低温下稳定性较好。包埋紫杉醇的PluronicF127/聚乳酸纳米粒子的释放曲线在前20h内呈现快速释放,此后表现为缓慢释放,约有20%的紫杉醇释放出来,MTT测试结果表明PluronicF127/聚乳酸嵌段共聚物具有很好的生物相容性。综合以上结果表明,PluronicF127/聚乳酸适合用作药物载体。BACKGROUND：Few reports about the stability of nanoparticles in the water and PBS have been published in China. OBJECTIVE：To investigate the stability of pluronic/poly（lactic acid） nanoparcticles in the water and PBS and feasibility of pluronic F127/poly（lactic acid） block polymer as drug carriers METHODS：Pluronic poly（lactic acid） nanoparcticles were prepared by a dialysis method. In vitro high performance liquid chromatography, and the cytotoxicity of pluronic F127/poly（lactic acid） block polymer was investigated by MTT. RESULTS AND CONCLUSION：Pluronic /poly（lactic acid） nanoparticles had better stability in the water than in the PBS. In addition, pluronic /poly（lactic acid） nanoparticles were influenced greatly by the temperature, which had a good stability in low temperature. At 37 ℃, pluronic/poly（lactic acid） nanoparticles had a greater change in the particle size. The release curve of paclitaxel-loaded pluronic F127/poly（lactic acid） nanoparticles showed a rapid release within 20 hours, and then presented with a slow release. Approximately 20% paclitaxel was released from the nanoparticles. MTT test results revealed that pluronic F127/poly（lactic acid） block polymer had a good biocompatibility. These findings indicate that pluronic/poly（lactic acid） nanoparticles can be used as drug carriers.

关 键 词：聚乳酸 PLURONIC F127 纳米粒子 药物载体 细胞毒性 紫杉醇 体外释放 生物相容性 缓释药物 嵌段共聚物 

分 类 号：R318[医药卫生—生物医学工程]