不同成分纳米银冲洗粪肠球菌离体根管感染的抗菌性能  

Antimicrobial efficacy of nano-silver endodontic irrigations for an in vitro model infected by Enterococcus faecalis in starvation

作　　者：陈建洪[1] 王萍[1] 彭萌[1] 唐倩[1] 文韵笙[1] 

机构地区：[1]中山大学附属第三医院口腔科,广东省广州市510630

出　　处：《中国组织工程研究》2012年第51期9533-9540,共8页Chinese Journal of Tissue Engineering Research

基　　金：广东省自然基金委员会项目(9151008901000074)~~

摘　　要：背景:牙髓根管治疗中有效的根管冲洗可明显减少根管系统内细菌及其产物,改善治疗效果及预后。目的:分析含醋酸氯已定或三氯生纳米银根管冲洗剂对饥饿期粪肠球菌离体根管感染模型的抗菌效果。方法:将45颗牙体完整、发育正常的人单根管前磨牙随机分为3组,建立饥饿期粪肠球菌离体根管感染模型,对感染模型使用Protaper手动器械进行根管预备至F3,同时分别使用含醋酸氯已定纳米银根管冲洗剂、含三氯生纳米银根管冲洗剂及次氯酸钠进行根管冲洗,采用菌落计数法计算根管预备冲洗前后感染根管内的菌落数。结果与结论:含醋酸氯已定或三氯生纳米银根管冲洗剂及次氯酸钠冲洗后根管内细菌数量均明显低于冲洗前(P<0.01)。含醋酸氯已定纳米银根管冲洗剂组与次氯酸钠冲洗组冲洗后的细菌数量低于含三氯生纳米银根管冲洗剂组(P<0.05),前两组间差异无显著性意义。表明含醋酸氯已定纳米银根管冲洗剂较含三氯生纳米银根管冲洗剂具有更强的抗菌效果。BACKGROUND：Effective root canal irrigation in root canal treatment can significantly reduce bacteria and their products within the root canal system, improving the treatment effectiveness and prognosis. OBJECTIVE：To evaluate the antimicrobial effects of two kinds of nano-silver endodontic irrigates containing chlorhexidine acetate or triclosan against Entercoccus faecalis in starvation in root canals. METHODS：Totally 45 infected premolars models used in the experiment were divided into three groups randomly, all of which were prepared with Protaper hand instruments to F3, and were irrigated with irrigations containing chlorhexidine acetate, triclosan and NaClO. Microbial growth was analyzed by turbidity of the culture medium, and then colony-forming units were counted on the plates. RESULTS AND CONCLUSION：There was a statistically significant difference in colony-forming units of Entercoccus faecalis before and after irrigation （P 0.01）. All of the three irrigations were antimicrobial. There was no significant difference in the antimicrobial effect between chlorhexidine acetate nano-silver and NaClO （P 0.05）. Chlorhexidine acetate nano-silver and NaClO showed a better antimicrobial effect than triclosan nano-silver, which was statistically significant （P 0.05）. These findings indicated that chlorhexidine acetate nano-silver, triclosan nano-silver and NaClO were antimicrobial, which could not eradicate Entercoccus faecalis in the root canals. Antimicrobial effects of chlorhexidine acetate nano-silver and NaClO were similar, which were better than triclosan nano-silver.

关 键 词：纳米银 根管冲洗 醋酸氯已定 三氯生 饥饿期粪肠球菌 次氯酸钠 平板计数法 生物材料 

分 类 号：R318[医药卫生—生物医学工程]