纳米细菌纤维素的细胞生物相容性  

Cellular biocompatibility of nano-bacterial cellulose

作　　者：李扬[1,2] 沈建成[2] 徐枫[2] 张华[1] 陈磊[3] 杨惠林[1] 施勤[1] 

机构地区：[1]苏州大学附属第一医院骨科,江苏省苏州市215006 [2]绍兴市中医院检验科,浙江省绍兴市312000 [3]中国科学院遗传和发育生物学研究所,北京市100190

出　　处：《中国组织工程研究》2012年第51期9541-9545,共5页Chinese Journal of Tissue Engineering Research

基　　金：江苏省骨科临床医学中心资助项目~~

摘　　要：背景:细菌纤维素是一种新型天然高分子材料,具有良好的三维网状结构和高持水性等独特性能。目的:观察天然高分子材料细菌纤维素与细胞共培养的生物相容性。方法:采用静置培养方案制备细菌纤维素支架,将处于对数生长期的C2C12细胞和L929细胞分别与细菌纤维素支架材料在体外共培养,采用扫描电镜观察材料微结构,倒置相差显微镜观察细胞生长及增殖情况,CCK-8比色法检测细胞增殖率。结果与结论:细菌纤维素具有精细的三维结构,纤维直径为纳米级别。C2C12细胞和L929细胞在细菌纤维素材料上可以很好地生长、增殖,细胞状态为正常的梭型,形态无明显变化;两种细胞在细菌纤维素材料上的相对增殖率均≥100%,细胞毒性为0级。说明细菌纤维素具有良好的生物相容性,对细胞黏附和增殖无影响。BACKGROUND：Bacterial cellulose is a new type of biological polymer materials, characterized by the advantages such as three-dimensional structure and high hydrophilicity. OBJECTIVE：To investigate the biocompatibility of the natural polymer bacterial cellulose by co-cultured with cells in vitro. METHODS：Bacterial cellulose scaffold was prepared with static culture program. Then the C2C12 cells and L929 cells in exponential phase of growth were co-cultured with bacterial cellulose in vitro. The morphology of bacterial cellulose scaffold was observed with scanning electron microscope. The growth and the proliferation of the cells were observed by using phase contrast microscope and the cell proliferation rate was measured by Cell Counting Kit-8 colorimetry. RESULTS AND CONCLUSION：Bacterial cellulose had a fine three-dimensional structure and the fiber diameter was in the nano-level. The C2C12 cells and L929 cells could in vitro grew and proliferate on the bacterial cellulose scaffold and showed fusiform shape; and their morphology did not changed significantly. The relative proliferation rates of C2C12 cells and L929 cells on the bacterial cellulose scaffold were ≥100%. The cytotoxicity grade of bacterial cellulose was 0. Bacterial cellulose has good biocompatibility which is benefit to cell adhesion and proliferation.

关 键 词：细菌纤维素 CCK-8 生物相容性 组织工程 生物材料 

分 类 号：R318[医药卫生—生物医学工程]