复合骨形态发生蛋白缓释材料修复兔桡骨缺损  

Bone morphogenic protein slow-release compound materials for repair of radial defects in rabbits

作　　者：崔旭[1] 马远征[1] 李大伟[1] 薛海滨[1] 

机构地区：[1]解放军第309医院骨科,全军骨科中心,北京市100091

出　　处：《中国组织工程研究》2012年第51期9558-9562,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81071454);项目名称:抗结核药物缓释/待骨材料缓释系统的研制~~

摘　　要：背景:在聚乳酸-聚羟基乙酸中加入β-磷酸三钙可调控其降解速率和强度,加载骨形态发生蛋白可增强材料的诱导成骨能力。目的:检验β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼/骨形态发生蛋白缓释材料修复兔桡骨节段性骨缺损的效果。方法:制作兔左侧桡骨12mm缺损模型,随机分4组,分别于骨缺损处植入β-磷酸三钙/聚乳酸-聚羟基乙酸材料、β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼缓释材料、β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼/骨形态发生蛋白缓释材料,并设置空白对照组(不植入任何材料)。结果与结论:术后12周时,β-磷酸三钙/聚乳酸-聚羟基乙酸材料组、β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼缓释材料组、β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼/骨形态发生蛋白缓释组骨缺损都得到较好修复,其中β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼/骨形态发生蛋白缓释组骨缺损修复效果最佳(P<0.05),空白对照组骨缺损未修复。表明β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼/骨形态发生蛋白缓释材料可很好修复兔节段性骨缺损。BACKGROUND：The degradation rate and strength of poly （lactic-co-glycolic acid） （PLGA） can be regulated by the addition of β-tricalcium phosphate （TCP）, and the load of bone morphogenetic protein （BMP） can promote the osteoinductive capacity. OBJECTIVE：To test the effect of slow-release PLGA/β-TCP/isoniazid （INH）/BMP material on the repair of segmental radial defects in rabbits. METHODS：Forty New Zealand rabbits were collected and a rabbit model of 12-mm left radial defects was established. The rabbits were randomly divided into four groups：group 1, group 2, group 3 and group 4. The former three groups were implanted PLGA/β-TCP material, PLGA/β-TCP/INH material and PLGA/β-TCP/INH / BMP material, respectively. The group 4 served as the blank control group （without implants）. RESULTS AND CONCLUSION：At week 12 after treatment, radial defects in the former three groups were all repaired well, and the PLGA/β-TCP/INH /BMP group obtained the best results （P 0.05）. The blank control group failed to restore radial defects. These results suggest that the slow-release PLGA/β-TCP/INH/BMP material can effectively repair the 12-mm long radial defects in rabbits.

关 键 词：聚乳酸-聚羟基乙酸 Β-磷酸三钙 骨形态发生蛋白 桡骨缺损 骨组织工程 

分 类 号：R318[医药卫生—生物医学工程]