骨髓间充质干细胞与膀胱脱细胞基质的生物相容性  

Biocompatibility of bone marrow mesenchymal stem cells with bladder extracellular matrix

作　　者：李欣慧[1] 廖文彪[1] 李永伟[1] 宋超[1] 杨嗣星[1] 

机构地区：[1]武汉大学人民医院泌尿Ⅱ科,湖北省武汉市430060

出　　处：《中国组织工程研究》2012年第51期9567-9573,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30872594)~~

摘　　要：背景:在尿路缺损修复中,骨髓间充质干细胞-膀胱脱细胞基质复合物有望形成上皮层、肌层及血管化。目的:观察骨髓间充质干细胞与膀胱脱细胞基质的生物相容性。方法:将第4代兔骨髓间充质干细胞按1.0×109L-1种植于同种异体兔膀胱脱细胞基质支架上,以单独培养骨髓间充质干细胞为对照。绘制两组细胞生长曲线图,对比观察两条生长曲线有无差异。结果与结论:骨髓间充质干细胞在膀胱脱细胞基质表面能够很好的贴附、分裂生长,且细胞生长曲线与对照组相似,说明骨髓间充质干细胞在膀胱脱细胞基质表面生长良好,相容性较好。BACKGROUND：In the process of urinary tract defect recondition, the composite of bone marrow mesenchymal stem cells-bladder extracellular matrix is expected to form the epithelium, muscularis and vascularization. OBJECTIVE：To observe the biocompatibility of bone marrow mesenchymal stem cells with bladder extracellular matrix. METHODS：Passage 4 rabbit bone marrow mesenchymal stem cells were seeded into bladder extracellular matrix of allogeneic with a density of 1.0×10 6 /mL. The separated cultured bone marrow mesenchymal stem cells were regarded as control. The growth curves of the two groups were drawn and the difference between them was evaluated. RESULTS AND CONCLUSION：Bone marrow mesenchymal stem cells could attach, mitosis and growth on the surface of bladder extracellular matrix, and the cell growth curve was similar with that of the control group. This indicated that the bone marrow mesenchymal stem cells that grow well on the surface of bladder extracellular matrix.

关 键 词：膀胱脱细胞基质 骨髓间充质干细胞 生物相容性 组织工程 尿路缺损 生物材料 

分 类 号：R318[医药卫生—生物医学工程]