重组人白细胞介素10抗家兔皮肤移植排斥反应中Toll样受体的变化  

Changes of Toll like receptors in the reaction of recombinant human interleukin-10 resisting rabbit skin grafting

作　　者：吕岩岩[1] 孙万邦[1] 李佳[1] 戴小波[1] 张磊[1] 王胜香[1] 陈富超[1] 罗军敏[1] 

机构地区：[1]遵义医学院珠海校区/贵州省免疫学研究生教育创新基地,广东省珠海市519041

出　　处：《中国组织工程研究》2012年第53期9980-9986,共7页Chinese Journal of Tissue Engineering Research

基　　金：贵州省教育厅自然科学基金重点培育资助项目(2008032);优秀人才省长基金资助项目(200607)~~

摘　　要：背景:现已认识到Toll样受体的活化与器官移植后排斥反应的关系十分密切,Toll样受体与移植免疫也逐渐成为研究的热点问题。目的:观察重组人白细胞介素10抗皮肤移植排斥反应中受体兔血清Toll样受体的变化。方法:制备兔背部缺损模型,将受体家兔分为6组,重组人白细胞介素10低、高剂量组、环孢素A低、高剂量组、联合用药组和生理盐水组,分别于移植前1d开始肌肉注射5,10μg/(kgd)重组人白细胞介素10,5,10mg/(kgd)环孢素A,5μg重组人白细胞介素10+5mg环孢素A,1mL/d生理盐水,连续用药10d。结果与结论:各组移植后Toll样受体4,7,8,9水平均较移植前升高,移植后14d达到高峰。移植后4,7d生理盐水组Toll样受体4水平高于联合用药组(P<0.05)。移植后7d生理盐水组Toll样受体7高于其他各组(P<0.05),Toll样受体8高于环孢素A高剂量组(P<0.05)。各组移植不同时间点Toll样受体9水平差异无显著性意义(P>0.05)。提示重组人白细胞介素10抗家兔皮肤移植反应中,Toll样受体4,7,8,9水平在皮肤移植后明显升高,当皮片排斥后达到高峰,之后逐渐下降,表明Toll样受体参与排斥反应过程。BACKGROUND： The researchers have recognized that there is a very close relationship between the activation of the Toll-like receptors and organ transplant rejection, and the Toll-like receptors and transplantation immunology have gradually become a hot topic. OBJECTIVE： To observe the changes of Toll-like receptors in the serum drawing from rabbits treated with recombinant human interleukin-10 after allogeneic skin grafting. METHODS： The rabbit back defect models were prepared, and the receptor rabbits were divided into six groups： low dose recombinant human interleukin-10 group, high dose recombinant human interleukin-10 group, low dose cyclosporine A group, high dose cyclosporine A group, joint group and normal saline group. The 5 and 10 μg/（kg d） recombinant human interleukin-10, 5 and 10 mg/（kg d） cyclosporine A, 5 μg recombinant human interleukin-10＋5 mg cyclosporine A and 1 mL/d normal saline were injected into the rabbits respectively from 1 day before grafting and lasted for 10 days. RESULTS AND CONCLUSION： The levels of serums Toll-like receptor 4, Toll-like receptor 7, Toll-like receptor 8 and Toll-like receptor 9 were increased after skin grafting compared with that before grafting and reached to the peak concentration on 14 days. At 4 and 7 days after grafting, the level of Toll-like receptors 4 in the normal saline group was higher than that in the joint group （P 0.05）. At 7 days after grafting, the level of Toll-like receptor 7 in the normal saline group was higher than that in the other five groups （P 0.05）, and the level of Toll-like receptor 8 in the normal saline group was higher than that in the high dose cyclosporine A group （P 0.05）. There was no significant differenceof Toll-like receptor 9 level in each two groups at different time points after grafting （P 0.05）. The levels of serum Toll-like receptor 4, Toll-like receptor 7, Toll-like receptor 8 and Toll-like receptor 9 were increased obviously after the rabbits skin grafting which have treated with re

关 键 词：重组人白细胞介素10 皮肤移植 环孢素A Toll样受体4 TOLL样受体7 Toll样受体8 TOLL样受体9 兔 免疫排斥 

分 类 号：R617[医药卫生—外科学]