栀子豉汤对自发性高血压大鼠AT1受体mRNA表达的影响  

Effects of Gardeniae and Sojae Praeparati Decoction(栀子豉汤) on the expression of AT1 receptor mRNA of spontaneously hypertensive rats

作　　者：李琛[1] 赵静[1] 冯薇[1] 王鑫国[1] 田义龙[1] 

机构地区：[1]河北医科大学中医学院,河北省实验动物重点实验室,石家庄050091

出　　处：《中药药理与临床》2012年第6期1-3,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河北省自然科学基金资助项目(C2009001061,H2012206019);河北省教育厅青年基金资助项目(2011178)

摘　　要：目的:观察栀子豉汤对自发性高血压大鼠(SHR)的血压和血管紧张素Ⅱ(AngⅡ)1型受体(AT1R)mRNA表达的影响,初步探讨其防治糖尿病心血管病变的可能机制。方法:选取SHR 50只,按血压随机分为模型对照组,栀子豉汤4 g生药/kg、8 g生药/kg、16 g生药/kg组及卡托普利组(2 mg/kg),采用鼠尾动脉间接测压法测定大鼠血压,逆转录-聚合酶链式反应(RT-PCR)检测主动脉及心肌组织AT1R mRNA的表达。结果:栀子豉汤8 g生药/kg及16 g生药/kg组治疗4周后,SHR血压明显降低,主动脉及心肌AT1R mRNA表达下调。结论:栀子豉汤可降低血压,下调主动脉及心肌组织AT1R mRNA表达,可能对糖尿病心血管病变的防治具有一定价值。To observe the effects of Gardeniae and Sojae Praeparati Decoction （GSPD） on blood pressure （BP） and the expression of Angiotensin II type 1 receptor （AT1R） mRNA of spontaneously hypertensive rats （SHR） and to preliminarily explore its possible mechanism of preventing diabetic cardiovascular disease. Methods： 50 SHR were randomly divided into model group, GSPD 4 g crude drug/kg, 8 g crude drug/kg, 16 g crude drug/kg group and captopril group （2 mg/kg） accord!ng to BP. The indirect tail-artery blood pressure measure- ment was used to measure BP, the expression of AT1R mRNA in aortic and myocardium tissue was detected by reverse transcription polymer- ase chain reaction （RT-PCR） method. Results： After GSPD 8 g crude drug/kg and 16 g crude drug/kg group treated for 4 weeks, the BP of SHR was decreased significantly, the expression of AT1R mRNA in aortic and myocardium was down-regulated. Conclusions： GSPD can de- crease BP, down-regulate the expression of AT1 R n1RNA in aortic and myocardium tissue, thus .GSPD may have potentially clinical values in the prevention and treatment of diabetic cardiovascular disease.

关 键 词：栀子豉汤 自发性高血压大鼠(SHR) AngⅡ1型受体(AT1R) 

分 类 号：R285[医药卫生—中药学]