二苯乙烯苷保护溶血性磷脂酰胆碱诱导血管内皮细胞损伤的机制研究  

2,3,4′,5-tetrahydroxystilbene-2-O-β-D glucoside protects against LPC-induced endothelial cell injury and mechanism of its action

作　　者：张颖[1] 张燕堂[2] 郑文[3] 岳雯[4] 晏子超 王家富[4] 商战平[4] 

机构地区：[1]泰山医学院放射学院,泰安271016 [2]泰山医学院校医院,泰安271016 [3]山东省交通医院检验科,济南250031 [4]泰山医学院病理生理学教研室,泰安271016

出　　处：《中药药理与临床》2012年第6期32-35,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：泰山医学院青年基金资助项目(No.2010ZRQN046);泰安市科技局资助项目(No.20123062)

摘　　要：目的:探讨二苯乙烯苷(TSG)对血管内皮细胞在溶血性磷脂酰胆碱(LPC)诱导下的保护作用及其机制。方法:体外培养人脐静脉血管内皮细胞(HUVECs),将TSG以10.0;1.0;0.1mol/L预先作用于HUVECs 1小时后,继续培养,再给予10mg/L的LPC作用于HUVECs共同孵育24小时,检测细胞的存活率、MDA、NO和ROS的变化。结果:以10mg/L浓度LPC的模型组与对照组比较结果显示,观测到细胞培养上清液中MDA含量显著性增加,而细胞培养上清液中NO含量明显降低,细胞的活性氧水平有所增加,细胞的存活率降低。实验组与模型组比较发现,以0.1μmol/L作为起效剂量,在0.1～10.0μmol/L浓度的范围内有效,并具有一定的浓度依赖关系。随着药物有效浓度的增加,细胞培养上清液中MDA含量显著性降低,NO含量显著性升高,细胞的活性氧水平则降低和细胞的存活率却显著性升高。结论:二苯乙烯苷能够通过抑制活性氧水平,增加NO的生成,增加细胞的存活,从而对LPC损伤的血管内皮细胞发挥保护作用。To observe the protection of 2,3,4, 5-tetrahydroxystilbene- 2-O-J3-D glueoside （TSG） agaist Lysophosphatidylehdine （LPC）- induced vascular endothelial cell injury and the effect of TSG on Malefic Dialdehyde （ MDA）, NO, and apoptosis. Methods： The third and fourth generation of human umbilical vein endothelial cells （HUVECs）, which were cultured in vitro and propagated, were used in the experi- ment. The cells were randomly divided into three groups： the control group; the modal group （TSG） ; the experimental group （TSG ＋ LPC）. The blank group was not adminidtered with any reagent. To establish Group TSG, LPC was administered to HUVECs on the basis of 10mg/L and in- cubated with the cells for 24h to induce endothelial cell injury; To establish Group TSG ＋ LPC, TSG was administered to HUVECs on the basis of 10.0, 1.0, 0. 1 p. mol / L lh before LPC was administered on the basis of 10mg/L and then the cells were incubated for 24h. Nexfly, the cell viability, ADMA, NO, and apeptosis rate were detected separately. Results： Compared with that of the control group, the cell culture su- pernatant of Group TSG ＋ LPC had significantly increased MDA and ROA while notably decreased NO and increased apoptosis as well as re- duced cell viability. Then compared with the Lt~ injury group, Group TSG ＋ LPC had significantly decreased MDA and ROS while notably in- creased NO and decreased apoptosis as well as remarkably elevated cell viability in the cell culture supernatant. Conclusion： Stilbene glyco- sides may protect LPC-injured vascular endothelial cells by inhibiting the expression of ADMA, enhancing NO production and meanwhile inhibi- ting apoptosis and increasing ceLl survival rate.

关 键 词：二苯乙烯苷 内皮细胞 溶血性磷脂酰胆碱 活性氧水平 丙二醛 一氧化氮 

分 类 号：R285[医药卫生—中药学]