氧化苦参碱的镇痛作用  

Analgesic effect of oxymatrine in different pain models

作　　者：姜静[1] 冯建伟[2] 陈靖[3] 苗兰英[3] 高沁怡[2] 杨丽[1] 

机构地区：[1]辽宁中医药大学基础医学院药理教研室,沈阳110847 [2]中国医科大学附属第一医院核医学科,沈阳110001 [3]辽宁中医药大学实验教学中心,沈阳110847

出　　处：《中药药理与临床》2012年第6期50-52,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金(青年科学基金)项目(81102901)

摘　　要：目的:探讨在不同的疼痛模型中,氧化苦参碱(Oxymatrine,OMT)的镇痛效果。方法:昆明小鼠随机分为空白组(生理盐水组),吗啡组,纳洛酮组及给药组,采用醋酸扭体法、热板法,观察给药后小鼠扭体次数(WT)、舔后足潜伏期(HPPT),同时注射纳洛酮观察其对氧化苦参碱镇痛作用的影响;采用部分结扎坐骨神经法(PSNL)建立模型,用up and down方法分别测结扎前、结扎后第3,7,14,21天小鼠手术侧后足底给药前后足底机械缩足反应阈值(MWT),观察氧化苦参碱(150 mg/kg)对神经性疼痛的影响。结果:氧化苦参碱(25～200 mg/kg)各剂量依赖性地减少醋酸所致的小鼠扭体次数,提高小鼠热刺激引起的痛阈,且以上镇痛作用不能被纳洛酮所拮抗;氧化苦参碱(150 mg/kg)还能提高部分结扎坐骨神经法手术组小鼠的机械缩足反应阈值。结论:氧化苦参碱对物理性疼痛、化学刺激性疼痛及神经性疼痛均有抑制作用。To explore the analgesic effect of oxymatfine （OMT） in different models of pain. Methods：Analgesia and dose-response of OMT, together with its sensitivity to naloxone, were studied through the acetic acid writhing test and the hot-plate test in mice. Writhing times in acetic acid stimulation and response latencies on hot-plate were examined. Neuropathic pain was established in mice using partial sci- atic nerve ligation （PSNL） model. Effects of OMT on mechanical allodynia induced by von-frey hairs were investigated using the up-and - down method in PSNL mice. Results：Intraperitoneal injection of OMT significantly reduced acetic acid-inducod writhing times （ P 〈0.01 ） and increased the response latency in hot-plate test （ P 〈0.01 ）. Analgesic effect of OMT was in a dose-dependent manner. In addition, this effect of OMT was not antagonized by naloxone. Administration of OMT showed anti-allodynic effects as shown by increasing the threshold to yon Frey hair-induced-mechanical stimulation in PSNL mice. Conclusion： OMT has analgesic effect on chemical, thermal and mechanical stimulation in different pain models.

关 键 词：氧化苦参碱 镇痛 疼痛模型 纳洛酮 

分 类 号：R285[医药卫生—中药学]