广藿香油体外抗呼吸道病毒效果研究  

Study on the effect of anti-respiratory viruses of Patchouli Oil in vitro

作　　者：魏晓露[1] 彭成[1] 万峰[1] 

机构地区：[1]成都中医药大学药学院,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都610037

出　　处：《中药药理与临床》2012年第6期65-68,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家“十一五”支撑计划重点项目(2009BA184B04)

摘　　要：目的:研究广藿香油体外抗流感病毒、呼吸道合胞病毒、腺病毒、柯萨奇病毒及单纯疱疹病毒的效果,为抗病毒性感冒新药研发提供依据。方法:建立流感病毒感染MDCK细胞、呼吸道合胞病毒和腺病毒感染Hep-2细胞、柯萨奇病毒感染Hela细胞模型和单纯疱疹病毒感染Vero细胞模型,以细胞病变效应(CPE)、噻唑蓝(MTT)法作为观察和检测指标,了解不同浓度药物体外抗病毒效果及药物对细胞的毒性,实验中分别以利巴韦林和阿昔洛韦作为对照药。结果:广藿香油对MDCK、Hep-2、Vero和Hela细胞的TC50浓度均为0.101mg/ml,TC0浓度为0.092mg/ml。广藿香油能明显抑制柯萨奇病毒和腺病毒所致的细胞病变,IC50分别为0.081mg/ml、0.084mg/ml,治疗指数(TI)分别为1.25、1.20;可抑制流感病毒,IC50为0.088mg/ml,TI为1.15;对呼吸道合胞病毒作用很低,IC50为0.092mg/ml,TI为1.10;对单纯疱疹病毒几乎没有抑制作用。结论:体外抗病毒实验广藿香油显示出抗柯萨奇病毒、抗腺病毒、抗甲型流感病毒和抗呼吸道合胞病毒的作用,但不具有抗单纯疱疹病毒的作用。Observing the effect of patchouli oil against influenza virus, respiratory syncytial virus, adenovirus, Coxsackie virus, herpes simplex virus in vitro. Provide the basis of developing new antiviral influenza drugs. Methods： Establish models of influenza virus infecting MDCK cell, respiratory syncytial virus and adenovirus infecting Hep-2 cell, Coxsaekie virus infecting Hela cell and herpes simplex virus in- fecting Vero cell respectively. Use CPE and MTr as the observation and detection methods, to understand the antiviral effect of different con- centration and drug toxicity to the ceils. We use fibavirin and A Silowe as control medicine in different experiment according to the virus sus- ceptibility. Results： The TC50 of Patchouli oil to MDCK, Hep-2, Vero and Hela ceils is 0. 101 mg/ml, the TC0 is 0.092mg/ml. Patchouli oil could inhibit the Coxsackie virus and adenovirus induced cell lesion obviously, the ICso is 0. 081mg/ml and 0. 084mg/ml respectively, the therapeutic index （TI） is 1.25 and 1.20 respectively; Patchouli oil could inhibit the influenza virus, the ICs0 is 0.088mg/ml, TI is 1.15; On the role of the respiratory syncytial virus is very low, the ICs0 is 0. 092mg/ml, TI is 1.10; there is nerely no inhibitory effect on herpes simplex virus. Conclusion： Patchouli oil show the ability of anti-Coxsackie virus, anti-adenovirus, anti-influenza A virus and anti-respiratory synevtinl vires excent anti-herues simnlex virus in vitro antiviral exneriment

关 键 词：广藿香油 抗病毒 流感病毒 呼吸道合胞病毒 腺病毒 柯萨奇病毒 单纯疱疹病毒 

分 类 号：R285[医药卫生—中药学]