白芍总苷在免疫性肝损伤小鼠药代动力学研究  

Pharmacokinetics of total glucosides of paeony in immunological liver injuried mice

作　　者：韩丽[1] 张玲非[1] 张肖玲[2] 王鑫国[3] 牛丽颖[3] 

机构地区：[1]河北医科大学第三医院,石家庄050051 [2]白求恩国际和平医院,石家庄050082 [3]河北医科大学中医学院河北省实验动物重点实验室,石家庄050091

出　　处：《中药药理与临床》2012年第6期69-71,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河北省自然科学基金资助项目(C2010000498)

摘　　要：目的:研究白芍总苷在免疫性肝损伤小鼠体内的药动学特点。方法:采用卡介苗加脂多糖尾静脉注射法建立小鼠免疫性肝损伤模型,HPLC法测定模型小鼠和正常小鼠灌胃给予白芍总苷(0.47g/kg、1.41g/kg、2.82g/kg)后15、30、60、90、120、150、180、240、360、480、720min血浆中芍药苷和芍药内酯苷浓度,根据药时曲线计算药动学参数,采用SPSS11.5软件分析各组各剂量间药动学参数的异同。结果:与正常组相比,模型组小鼠体内白芍总苷的Tmax明显提前,t1/2明显延长,Cmax、AUC0-t和AUC0-∞显著增大;各剂量间白芍总苷的Tmax和t1/2没有差异,芍药苷剂量与Cmax、AUC0-t和AUC0-∞有一定的相关性。结论:免疫性肝损伤小鼠对白芍总苷的吸收速度较正常小鼠加快,吸收量较大,消除减慢。To study pharmacokineties of total glucosides of paeony （TGP） in immunological liver injuried mice. Methods： BCG and LPS caudal intravenous injection was used to establish immunological liver injuried mice. TGP （0.47, 1.41,2.82 g/kg） was orally admin- istered to normal and model animals. With HPLC, the concentration of Paeoniflofin and Albiflofin in plasma at times of 15, 30, 60, 90, 120, 150, 180,240,360,480 and 720 rain after dosing was determined. The parameters were calculated according to concentration-time curve. SPSS 11.5 was used to analyze dates between groups. Results： Compared with normal group, Tmax of TGP in model group signifi- cantly advanced, tl,,2 prolonged, Cmax, AUCo.t and AUC0.~ increased; between dosages, Tmax and tl/2 of TGP hadnt difference, and dosage of Paeonitlofin with Cmax, AUC0.t and AUC0.5 had determinate correlation. Conclusions ： TGP in immunological liver injuried mice was ab- sorbed more rapidly than normal mice, absorbed dose larger, and eliminated more slowly.

关 键 词：白芍总苷 药代动力学 免疫性肝损伤模型 芍药苷 芍药内酯苷 高效液相色谱法 

分 类 号：R285[医药卫生—中药学]