黄精多糖干预长春新碱诱导的骨髓基质细胞生长抑制及凋亡  

Effect of polygonatum sibiricum polysaccharides on preventing from the suppression and apoptosis of bone marrow stromal cells induced by vincristine

作　　者：胡微煦[1] 文珠[2] 戎吉平[2] 俞火[2] 胡国柱[3] 

机构地区：[1]复旦大学附属肿瘤医院,上海200032 [2]江西省医学科学研究所,南昌330006 [3]江西省人民医院临床医学研究所,南昌330006

出　　处：《中药药理与临床》2012年第6期79-82,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:探讨黄精多糖(PSP,polygonatum sibiricum polysaccharides)干预长春新碱(VCR)诱导的小鼠骨髓基质细胞(BMSCs)生长抑制及凋亡的作用。方法:①PSP浓度(500、1000、2000、4000mg/L)及VCR浓度(2.5、5、10、15mg/L)分别与骨髓细胞共同培养14d,应用MTT法测定BMSCs增殖。②PSP(500、1000、2000、4000mg/L)与骨髓细胞预培养2h后再分别加入终浓度为5mg/L的VCR继续培养14d,应用MTT法测定BMSCs增殖。③BMSCs培养14d后再加入VCR(2.5、5、10、15mg/L)继续培养24h,经Annexin V/PI双染流式细胞术(FACS)测定细胞凋亡。④PSP(500、1000、2000、4000mg/L)与BMSCs培养14d后再加入5mg/LVCR继续培养24h,经Hoechst 33342荧光染色及Annexin V/PI双染流式细胞术(FACS)测定细胞凋亡。结果:①PSP(1000、2000、4000mg/L)与骨髓细胞培养14d后OD值(3.80、4.48、4.05)均高于正常对照组(3.70),其中PSP 2000mg/L组OD值差异具有显著性(P<0.05);VCR(5、10、15mg/L)组OD值(2.36、1.83、1.67)均显著低于正常对照组(3.70)。②骨髓细胞与PSP孵育2h后再加入5mg/L VCR继续培养14d,PSP 2000mg/L+VCR 5mg/L组OD值(3.51)与VCR 5mg/L组OD值(2.62)比较差异有显著性。③BMSCs培养14d再加VCR继续培养24h后FACS测定VCR(5mg/L、10mg/L、15mg/L)组凋亡率分别为31.12%、39.37%、64.10%,与正常对照组(7.70%)比较差异有显著性。④PSP与BMSCs培养14d再加入5mg/L VCR继续培养24h后,FACS测定PSP(1000mg/L、2000mg/L和4000mg/L)组凋亡率依次为27.77%、24.33%和25.20%,与凋亡诱导组(35.93%)比较差异均有显著性;Hoechst 33342检测,PSP(2000mg/L、4000mg/L)组凋亡率为29.0%和29.67%,与凋亡诱导组(40.33%)比较差异均有显著性。结论:黄精多糖可促进BMSCs增殖,有效地阻止长春新碱对BMSCs生长的抑制及凋亡作用。To investigate the effect of PSP （polygnnatum sibiricum polysaccharides） on preventing from the suppression and apeptosis of mice bone marrow stromal cells （BMSCs） induced by VCR （Vincristine）. Methods： The BMSCs were cultured with PSP （500,1000, 2000,4000rag/L） or VCR （2.5, 5, 10, 15 mg/L ） for 14 days, respectively and the BMSCs proliferation was determined by MTr. The BMSCs were pretreated with PSP （500,1000,2000,4000mg/L） for 2 hour, then cultured with VCR（5 mg/L） for 14 days, and the BMSCs proliferation was determined by MTY. After the BMSCs were cultured for 14 days, then VCR （2.5, 5, 10, 15 mg/L） was added to culture for 24 hours, respectively, and cell apoptosis was determined by FACS with Annexin V/PI. The BMSCs were cultured with PSP （500,1000, 2000,4000rag/L） for 14 days, then VCR（5 rag/L） was added to culture for 24 hours, respectively, and cell apeptosis was determined by Hoechst 33342 fluorescent staining and FACS with Annexin V/PI. Results： After the BMSCs were cultured with PSP （ 1000,2000,4000rag/ L） for 14 days, the OD value of BMSCs were 3.80,4.48,4.05, and it was significantly higher in the PSP（200Omg/L） than in the normal control group （3.70） （ P 〈 0.05）. In the VCR groups （5,10,15 rag/L） the BMSCs grew slower along with VCR concentration increasing, and the OD value was significantly lower in the VCR groups （ 2.36,1.83,1.67 ） than in the normal control group （3.70） in 14th day （ P 〈 0.05 ）. After the BMSCs were pretreated with PSP for 2 hours, then cultured with VCR（5.0mg/L） forl4days, the OD value of BMSCs was signifi- cantly higher in the PSP（2000mg/L） （3.51 ） than in the V CR group（5mg/L） （2.62）（ P 〈 0.05 ）. After the BMSCs were cultured for 14 days, VCR（5, 10 or 15 rag/L） was added to culture and continued to be cultured for 24 hours, the apeptosis rates of BMSCs were signifi- candy higher in the VCR groups （31.12%, 39.37%, 64.10% ） than in the normal control group（7.70% �

关 键 词：黄精多糖 长春新碱 骨髓基质细胞 增殖 凋亡 

分 类 号：R285[医药卫生—中药学]