秦艽对四氯化碳致小鼠急性肝损伤的保护作用  

Protective effect of Radix Gentiana Macrophylla on acute liver injury induced by CCl_4 in mice

作　　者：康宏杰 张霞[2,3,4] 侯延辉[2,3,4] 吴洋[2,3,4] 徐力生[2,3,4] 孙磊[2,3,4] 

机构地区：[1]宁夏启元药业公司,银川750011 [2]宁夏医科大学药学院 [3]宁夏回药现代化工程技术研究中心 [4]宁夏医药研究所,银川750004

出　　处：《中药药理与临床》2012年第6期98-100,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：宁夏回族自治区卫生厅重点科研计划项目(编号:2009025);宁夏自然科学基金项目(编号:NZ11101)

摘　　要：目的:研究秦艽对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法:腹腔注射10%CCl4花生油溶液引起小鼠急性肝损伤,以联苯双酯为阳性对照,灌胃给药7天,测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和肝组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及脏器重量,并做病理切片,观察肝组织病理改变。结果:同模型组相比较,秦艽水提取物高、中、低剂量(8,4,2 g/kg)组均能降低CCl4所致急性肝损伤小鼠血清ALT、AST活性(P<0.01,P<0.05),降低肝脏MDA的含量,增强SOD活性(P<0.01,P<0.05);病理学切片显示,秦艽提取物能明显减轻模型动物肝脏病变程度。结论:秦艽水提物对CCl4所致小鼠急性肝损伤具有保护作用。To observe the protective effoct of Radix C, anfiana Maerophylla Pall on acute liver injury induced by CCI4 in mice. Methods： 60 mice were randomly divided into six groups： control group, model group, positive group （ bifendate）, and Radix Gentiana Maerophylla Pall extract high, medium and low dose treatment group. They were administered with NS, and three doses of Radix Gentiana Maerophylla Pall ex- tract were ig administrated to the models once daily for seven days. Acute hepatic in jury was induced by injecting 10 ml/kg 10% peanut oil solution of carbon tetraehloride. After the last administration,all groups except the normal group were injected with 10% CCI4 （ 10 ml/kg） peanut oil solution to induce acute liver injury model. Four biochemical indexes were detected,including ALT, AST, MDA and SOD. Patho- logical changes in liver were also investigated. Result： Compared with model group, serum AST, ALT in Radix Gentiana Macrophylla Pall extract high, medium and low dose（8,4,2 g/kg） groups reduced significantly（P 〈 0.01 or P 〈 0.05）, as well as MDA content decreased significantly, SOD increased（ P 〈 0.01 or P 〈 0.05 ） ; Histopathologieal study showed that the Radix Gentiana Maerophylla Pall extract have a good proteetive effect for the liver of mice. Conclusion： Radix Gentiana Maerophylla Pall extract has a protective effect on the carbon tetra- chloride induced acute liver injury in mice.

关 键 词：急性肝损伤 小鼠血清 保护作用 四氯化碳 秦艽 血清丙氨酸氨基转移酶 肝组织病理改变 天冬氨酸氨基转移酶 

分 类 号：R285[医药卫生—中药学]