宁夏枸杞叶总黄酮提取和纯化方法的优化  

Optimization of an Extraction and Purification Method of Total Flavonoids from Lycium barbarum Leaves

作　　者：马婷婷[1,2] 王忠忠[1] 牛东玲[1] 

机构地区：[1]宁夏大学生命科学学院,银川750021 [2]银川能源学院生物与农业工程系,银川750100

出　　处：《植物科学学报》2012年第6期644-650,共7页Plant Science Journal

基　　金：宁夏大学自然科学基金项目(NDZR10-3);国家科技支撑计划项目(2009BAI72B03)

摘　　要：采用L9(34)正交设计,以黄酮含量为指标,确定的宁夏枸杞叶总黄酮的最佳超声提取方法为:30倍70%乙醇超声提取50 min,超声功率为250 W;纯化方法条件为:5倍柱体积70%乙醇洗脱,浓度为4 mg/mL、pH值5.0的上样液,上样速度和洗脱流速均为1 mL/min。并以吸附率、解吸率、回收率等作为考察参数,对提取和纯化方法进行了验证。实验结果表明,该方法简便节时、易操作且毒害小,各项变异系数低。The content of total flavonoids as a evaluating factor and the extracting method of total flavonoids from Lycium barbarum leaves were investigated with an L9（34） orthogonal experiment.The optimum conditions were alcohol concentration 70%,ultrasonic time 50 min,ratio of liquor and material 30 ∶1,and ultrasonic wave power 250 W.The optimum conditions for purification were： addition of 4 mg/mL of the sample with pH 5.0,elution with 5.0 multiple column volume of 70% ethanol,sample flowing velocity 1 mL/min,and eluting velocity 1 mL/min.The extraction and purification methods were studied using adsorption capacity,desorption capacity,and recovery as the investigation parameters.Results showed that the method was superior to other methods due to its simplicity,low toxicity,and low variation coefficient.

关 键 词：宁夏枸杞叶 黄酮 大孔树脂 提取和纯化 正交设计 

分 类 号：Q946.8[生物学—植物学]