三七总皂甙对缺氧缺血性脑损伤新生鼠内源性神经干细胞增殖分化的影响  

Effect of panax notoginseng saponin on proliferation and differentiation of endogenous neural stem cells in neonatal rats with hypoxic-ischemia brain damage

作　　者：雷勋明[1] 陈全景[1] 张晓芬[1] 陈少军[1] 

机构地区：[1]湖北医药学院附属东风医院儿科教研室,湖北十堰442008

出　　处：《中国妇幼保健》2013年第4期684-687,共4页Maternal and Child Health Care of China

摘　　要：目的:观察三七总皂甙对缺氧缺血性脑损伤(HIBD)新生鼠内源性神经干细胞增殖分化的影响,探讨其促进神经发生的作用。方法 :将72只7日龄新生SD乳鼠随机分为假手术组、HIBD组及HIBD的三七总皂甙治疗组,用改良的Rice-Vannucci法制作HIBD模型。注射三七总皂甙干预治疗,用5一溴脱氧尿苷(BrdU)标记处于增殖状态的神经干细胞,免疫单标及双标免疫荧光技术观察三七总皂甙对HIBD海马区BrdU免疫活性及BrdU/NSE(Neuron specific enolase,NSE)、BrdU/GFAP(Glial fibrillary acidic protein,GFAP)双标免疫活性的影响,并计数分析。结果:HIBD的海马区存在BrdU阳性细胞分布;应用三七总皂甙的HIBD海马区BrdU阳性细胞数及双标阳性细胞明显增多(P<0.01)。结论:三七总皂甙能诱导HIBD新生鼠内源性神经干细胞增殖、分化,提示其具有促进神经发生的能力。Objective： To observe the effect of panax notoginseng saponin （PNS） on proliferation and differentiation of endogenous neural stern cells in neonatal rats with hypoxie - isehemia brain damage （ HIBD ） . Methods ： Seventy - two neonatal SD rats aged seven days old were randomly divided into Sham operation group, HIBD group, and PNS group, modified Rice - Vannucci method was used ｜o es- tablish HIBD models. The rats were treated with injection of PNS, 5 - bromodeoxyuridine （BrdU） was used to label neural stem ceils at pro- ]iferative state, immunohistochemieal single staining and double - immunofluorescence technique were used to observe the effect of PNS on BrdU immunoreaetivity in hippoeampus of HIBD rats and BrdU/neuron specific enolase （NSE） , BrdU/glial fibrillary acidic protein （GFAP） dual- labeling immunoreactivities, then the results were counted and analyzed. Results： Positive BrdU cells existed in hippocampus of HIBD rats ; the numbers of positive BrdU cells and dual - labeling positive cells in hippocampus of HIBD rats treated with PNS increased sig- nificantly （ P 〈 O. O1 ） . Conclusion： PNS can induce the proliferation and differentiation of endogenous neural stem ceils in HIBD rats, which indicates that it may have the ability of promoting neurogenesis.

关 键 词：缺氧缺血性脑损伤 三七总皂甙 干细胞 细胞增殖 

分 类 号：R722[医药卫生—儿科]