乙酰化白藜芦醇保护海水淹溺型急性肺损伤的机制研究  

Pathogenesis of acetylated resveratrol in the protection of seawater aspiration - induced lung inury

作　　者：马李杰[1] 赵诣林[1] 刘雪英[2] 金发光[1] 

机构地区：[1]解放军第四军医大学唐都医院呼吸科,陕西西安710038 [2]解放军第四军医大学药学系药物化学教研室

出　　处：《中国急救医学》2013年第1期74-76,100,共4页Chinese Journal of Critical Care Medicine

基　　金：基金项目：国科金面上项目（81270124）

摘　　要：目的观察海水淹溺型急性肺损伤（SWD—ALI）的发病机制，验证乙酰化白藜芦醇通过抑制核因子一KB（NF—KB）的表达减轻SWD—ALI。方法32只大鼠分为正常对照组、模型组、乙酰化白藜芦醇预处理组和乙酰化白藜芦醇对照组。模型组和乙酰化白藜芦醇预处理组气管内注入4mL／kg海水，4h后检测肺损伤指标，并用ELISA法检测肺组织中NF—KB的表达量。结果与正常对照相比，模型组大鼠出现明显肺损伤，并伴有炎性细胞浸润和炎性因子的分泌，同时肺组织中NF—KB表达量增高；乙酰化白藜芦醇预处理组吸入海水后肺部损伤明显减轻，NF—KB表达受到抑制。结论NF～KB的促炎机制参与SWD—ALI的发生，乙酰化白藜芦醇能够通过抑制NF—KB表达减轻SWD—ALI。Objective To observe the mechanism of seawater aspiration - induced lung injury and the protective effect of acetylated resveratrol. Methods 32 rats were randomly divided into normal control group, model group, acetylated resveratrol pre - treated group and acetylated resveratrol control group. Rats from model group and acetylated resveratrol pre - treated group were subjected to seawater aspiration. All the rats were sacrificed four hours later, nuclear factor - KB（ NF - KB）and indicators of lung injury were detected. Results Seawater aspira;ion led to lung injury with the characteristics of inflammatory eeUs infiltration and inflammatory factor secretion, acetylated resveratrol can suppress the expression of hrF - KB and protect lung tissue from seawater aspiration. Conclusion NF - KB plays an important role in the onset and development seawater aspiration - induced lung injury, and acetylated resveratrol can protect lung tissue by regulating the expression of NF - KB.

关 键 词：海水 急性肺损伤(ALI) 核因子-KB(NF—KB) 乙酰化白藜芦醇 

分 类 号：R563[医药卫生—呼吸系统]