检测兔出血症病毒荧光定量方法的建立与应用  

Establishment and application of real-time PCR method for detection of rabbit haemorrhagic disease virus

作　　者：刘行波[1,2] 原东伟[2] 刘家森[2] 郭冬春[2] 姜骞[2] 林欢[2] 司昌德[2] 崔玉东[1] 曲连东[2] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室实验动物研究室,哈尔滨150001

出　　处：《黑龙江畜牧兽医》2013年第2期89-91,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：兽医生物技术国家重点实验室开放基金项目(SKLVBD201002)

摘　　要：为了研究兔出血症病毒(Rabbit hemorrhagic disease virus,RHDV)在实验动物兔和野兔中的发生情况并能更精确检测该病毒,试验采用建立的SYBR Green荧光定量方法对10只黑龙江省各单位送检的实验动物兔和5只哈尔滨郊县疑似病例的脏器样品进行了检测。结果表明:该方法有较好的灵敏度和特异性,能够用于兔出血症病毒的检测。To detect Rabbit hemorrhagic disease virus （RHDV） more precisely and learn the occurrences of this virus between experimental rab- bits and hare. A real - time PCR method with SYBR Green was established for the detection of ten experimental rabbits from the various units in Heilongjiang Province for inspection and 5 samples of suspected cases in hares. The results indicated that the method had good sensitivity and specificity, and could be used to detect RHDV.

关 键 词：兔出血症病毒(RHDV) VP60 REAL-TIME PCR 隐性感染 

分 类 号：S829.1[农业科学—畜牧学]