吴茱萸提取物乳膏镇痛作用及其机制探讨  

Analgesic Effects of Evodia Extract Cream and Its Mechanisms in Mice

作　　者：王婷[1] 陈菲[1] 李溯[1] 丁劲松[1] 

机构地区：[1]中南大学药学院,长沙410013

出　　处：《中国药学杂志》2013年第2期106-110,共5页Chinese Pharmaceutical Journal

摘　　要：目的研究吴茱萸提取物乳膏镇痛作用及其机制。方法以昆明小鼠为模型动物,采用热板法和福尔马林致痛法研究吴茱萸提取物乳膏镇痛作用;采用酶联免疫法测定降钙素基因相关肽及前列腺素E2的含量,HE染色法考察疼痛部位组织炎性细胞浸润程度,探讨吴茱萸提取物乳膏在体镇痛机制。结果 0.1%～0.4%吴茱萸提取物乳膏能缓解热所致小鼠疼痛(P<0.05),对福尔马林所致两相疼痛均有明显镇痛作用(P<0.05),并呈载药量依赖性。酶联免疫法结果表明,0.2%吴茱萸提取物乳膏能明显降低皮下注射福尔马林3 min后疼痛部位皮肤中降钙素基因相关肽表达量(P<0.05),且降钙素基因相关肽量随时间的延长而减少(P<0.05);但0.1%～0.4%吴茱萸提取物乳膏对血浆中降钙素基因相关肽和前列腺素E2含量无显著影响,对皮肤中前列腺素E2含量无影响。切片结果证实,0.1%～0.4%吴茱萸提取物乳膏能显著降低福尔马林引起的炎症细胞浸润程度(P<0.05)。结论局部施用吴茱萸提取物乳膏对小鼠有镇痛作用,并呈载药量依赖性,其镇痛机制可能与耗竭并阻断降钙素基因相关肽传导并协同抗炎作用有关。OBJECTIVE To study the analgesic effects of Evodia extract cream and its mechanisms. METHODS Kunming mice were used as model animals, and hot plate test and formalin test were adopted in analgesia experiments. ELISA test was used to detect the contents of CGRP and PGE：. In addition, the tissue section technique was used to investigate the extent of inflammation cell infiltration in the area of pain. RESULTS Evodia extract cream with a drug loading ranging from 0. 1% to 0. 4% could relieve pain induced by noxious heat （ P 〈 0.05 ）, and demonstrated significant analgesic effects in the mice formalin test （ P 〈 0. 05 ） in a dose-de- pendent manner. Results from ELISA test showed that Evodia extract cream with a drug loading of 0. 2% significantly decreased the expression of CGRP in the pain zone of skin （ P 〈 0. 05 ） 3 min after subcutaneous injection of formalin （0. 2% EE） , and the expression of CGRP showed significant reduction over time （ P 〈 0. 05 ）. But Evodia extract cream with a drug loading ranging from 0. 1% to 0. 4% had no significant effects on the contents of CGRP and PGE2in plasma or the content of PGE2in mice skin. Histological section results indicated that the content of inflammation cell infiltration induced by formalin was decreased significantly after topical administration of Evodia extract cream with a drug loading ranging from 0. 1% to 0. 4% （ P 〈 0. 05 ）. CONCLUSION Topical administration of Evodia extract cream has significant dose-dependent analgesic effects. These findings suggest that the analgesic mechanism of Evodia extract cream is most likely related with exhausting the CGRP and interdicting its conduction and coordinating with anti-inflammatory effect.

关 键 词：吴茱萸 乳膏 局部给药 镇痛 

分 类 号：R965[医药卫生—药理学]