利用重组自交系定位棉花第22染色体短臂的纤维品质性状的QTL  

QTL Mapping of Fiber Quality Traits for Elite Hybrid Derived-RILs on Chromosome 22sh in Cotton

作　　者：姚金波[1] 陈伟[1] 张永山[1] 郭香墨[1] 褚丽[1] 刘海菊[1] 

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000

出　　处：《棉花学报》2013年第1期24-29,共6页Cotton Science

基　　金：国家科技支撑计划(2011BAD35B05-1);国家科技重大专项(2011ZX08005-003)

摘　　要：以TM-1的染色体片段代换系CSB22sh和TM-1杂交,构建了包含104个家系的重组自交系(RILs)群体。利用74对具有多态性位点的引物进行检测,构建了包含61个多态性位点,长度为76.93 cM的遗传图谱,平均标记间距离1.26 cM。利用此遗传图谱结合重组自交系群体4个环境下的5个纤维品质性状进行QTL定位,共定位出12个QTLs,解释性状表型变异的2.45%～21.11%;在1,2,3,4个环境中同时检测到的QTLs分别有9,1,1,1个。而利用4个环境平均值进行联合分析定位出个4个QTLs,纤维长度和纤维整齐度的QTLs均为2个,解释性状表型变异的14.37%～19.97%,并且纤维长度和整齐度的QTL在相同的位置。多环境下检测到的QTL可能对标记辅助选择有实际应用价值。A population of 104 recombinant inbred lines （RILs） was developed by single seed descended from the cross of upland cotton （Gossypium hirsutum L.） TM-1 and its substitution line, CSB22sh. Agenetic linkage map consisting of 61 loci and cover- ing 76.93 cM was constructed using SSR markers, with the average distance of 1.26 cM between two markers. Composite inter- val mapping was used to identify the quantitative trait loci （QTL） in RILs, 12 QTLs for fiber quality were independently detected in four environments, whereas 4 QTLs were found in joint analysis. These QTLs explained 2.45%-21.11% of the phenotypic variance. There were 9, 1, 1 and 1 QTLs simultaneously significant in one, two, three and four environments, respectively. There were two fiber length and two fiber uniformity QTLs in joint analysis, which explained 14.37% - 19.97% of the phenotypic vari- ance and in same position respectively. Notably, a stable fiber length QTL qF1-22-2 was detected both in joint analysis and in four environments of separate analysis, which might be of special value for marker-assisted selection.

关 键 词：重组自交系 置换系 遗传连锁图谱 纤维品质性状 QTL 

分 类 号：S562[农业科学—作物学]