转Cry1Ac+Cry2Ab抗虫棉对3种害虫的存活、生长发育及中肠相关酶活性的影响  

Effects of Transgenic Cry1Ac plus Cry2Ab Cotton on Pest Survival,Growth and Enzymes Activity of Three Kinds of Pests

作　　者：雒珺瑜[1] 崔金杰[1] 张帅[1] 辛惠江[1] 王春义[1] 

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000

出　　处：《棉花学报》2013年第1期68-72,共5页Cotton Science

基　　金：农业部转基因生物新品种培育重大专项(2013ZX08011-002)

摘　　要：以转Cry1Ac基因棉花和常规非转基因棉花为对照,系统研究了转Cry1Ac+Cry2Ab基因棉花对棉铃虫、斜纹夜蛾、甜菜夜蛾的存活、生长发育及中肠酶活性的影响。研究结果表明,与常规棉对照相比,转Cry1Ac+Cry2Ab基因棉花和转Cry1Ac基因棉花叶片对3种害虫的慢性毒性差异较大,对每种害虫的抗性程度也有一定的差异;饲喂转Cry1Ac+Cry2Ab基因棉花和转Cry1Ac基因棉花叶片的3种害虫幼虫生长发育进度明显缓慢、体重明显减轻,相关酶活性也有不同程度的变化,但每种酶的变化有一定的差异。Using the transgenic CtylAc cotton and non-transgenic cotton as control, the effects of transgenic CtylAc plus Cry2Ab cotton on survival, growth and enzymes activity of three types of pests （Helicoverpa armigera Htibner, Spodoptera liture and Spodoptera exigua Htibner ） were studied. The results show that, the difference of chronic toxicity to three types of pests fed with leaves from different cotton varieties is significant, and resistance of every type cotton varieties to these pests is different too. Compared to the conventional variety CCRI 49, the growth speed of three pests fed with transgenic CrylAc＋Cry2Ab gene cotton and transgenic CrylAc cotton is slower, and the weight is lighter. Meanwhile enzymes activity change with different de- grees too, but the changes of each enzyme activity have some difference.

关 键 词：转Cry1Ac+Cry2Ab棉花 抗虫性 生长发育 酶活性 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]