16份马蔺种质材料苗期耐盐性评价  

Evaluation of salt tolerance for 16 Iris lactea var. chinensis accessions at seedling stage

作　　者：毛培春[1] 田小霞[1] 孟林[1] 

机构地区：[1]北京市农林科学院北京草业与环境研究发展中心,北京100097

出　　处：《草业科学》2013年第1期35-43,共9页Pratacultural Science

基　　金：北京市自然科学基金项目(6102013);北京市财政专项(KJCX201101003)

摘　　要：采用温室NaCl盐分胁迫试验方法,设置0、0.4%、0.8%、1.2%4个NaCl质量分数梯度,对来自我国北方4个省区16份马蔺(Iris lactea var.chinensis)种质材料的生理生化指标进行测定,并对其苗期耐盐性进行评价,筛选耐盐性较强的种质材料,为马蔺种质资源的开发利用和耐盐新品种的选育奠定基础。结果表明,随盐胁迫质量分数的增加,叶片相对含水量呈下降趋势,相对电导率、丙二醛和游离脯氨酸含量呈上升趋势,而叶绿素含量呈先升后降的趋势,在胁迫质量分数0.4%时达峰值。采用系统聚类法将16份马蔺种质材料耐盐性分为较强、居中和较弱三大类,其中耐盐性较强的8份(BJCY-ML001、BJCY-ML016、BJCY-ML012、BJCY-ML023、BJCY-ML007、BJCY-ML011、BJCY-ML024、BJCY-ML029)、耐盐性居中的4份(BJCY-ML006、BJCY-ML005、BJCY-ML031、BJ-CY-ML013)和耐盐性较弱的4份(BJCY-ML018、BJCY-ML020、BJCY-ML021、BJCY-ML035),采用标准差系数赋予权重法对其耐盐能力大小进行排序,BJCY-ML007耐盐能力最强,BJCY-ML035耐盐能力最弱。In order to select new varieties with high salt tolerance and to provide scientific basis for their development and utilization, 16 accessions of Iris lactea var. chinensis collected from four provinces in China were treated with four NaCl concentration solutions： 0, 0. 4%, 0. 8% and 1. 2% at the seedling stage in a greenhouse. The physiological and biochemical indexes, including relative water content, rela-tive conductivity rate, MDA content, contents of free proline and chlorophyll in their leaves under NaCl salt stress were measured and analyzed. The results showed that with the increasing of NaCl concentra-tions, relative water content of leaves decreased, while contents of MDA and free proline increased. Con-tent of chlorophyll increased first and then decreased, and reached the peak at 0.4% NaCl concentration. According to the results of the cluster analysis of salt tolerance, 16 accessions could be divided into three groups, e. g. the stronger salt tolerance （BJCY-ML001, BJCY-ML016, BJCY-ML012, BJCY-ML023, BJCY-ML007, BJCY-ML011, BJCY-ML024 and BJCY-ML029）, the medium （BJCY-ML006, BJCY-ML005, BJCY-ML031 and BJCY-ML013） and the weaker salt tolerance （BJCY-ML018, BJCY-ML020, BJCY-ML021 and BJCY-ML035）. Furthermore, the salt tolerance of 16 accessions was ordered using the standard deviation coefficient allocation weighted method, BJCY-ML007 and BJCY-ML035 were the acces-sions with the strongest and the weakest salt tolerance, respectively.

关 键 词：马蔺 苗期 耐盐性 

分 类 号：S682.19[农业科学—观赏园艺]