内毒素对肉鸡肝脏线粒体复活体Ⅰ活性的影响及氨基胍的保护效应  

Effect of Endotoxemia on Activity of ComplexⅠ of Mitochondia in Liver and Protection of Aminoguanidine in Broilers

作　　者：石冬梅[1] 王岩[1] 向瑞平[1] 皇甫和平[1] 张华[1] 唐兆新[2] 徐家华[2] 王友令[2] 张胜[2] 

机构地区：[1]郑州牧业工程高等专科学校,河南郑州450011 [2]华南农业大学兽医学院,广东广州510642

出　　处：《中国畜牧兽医》2013年第1期122-124,共3页China Animal Husbandry & Veterinary Medicine

基　　金：中国博士后科学基金(2003033411);河南省重点科技攻关(82102130014);河南省高校杰出科研人才创新工程(2004KYCX019)项目资助

摘　　要：本研究采用30日龄黄羽肉鸡90只,随机分成内毒素处理组(LPS组,5mg/kg)、氨基胍治疗组(AG+LPS组)和生理盐水对照组。每组分别在处理后1、3、5、7和9h时间点杀鸡取样,取肝脏组织匀浆,提取线粒体,分别检测肝脏线粒体复活体Ⅰ活性。试验结果显示,LPS在各时间段显著降低了肉鸡肝脏线粒体复合体Ⅰ的活性(P<0.05)。注射AG 1h时,肉鸡肝脏线粒体复合体Ⅰ的活性未发生变化(P>0.05),3h时却显著增加(P<0.05);5、7h时,趋于正常(P>0.05);9h时,显著降低(P<0.05)。由此推论,LPS诱导了线粒体的损伤,引起了线粒体内的复活体Ⅰ活性的紊乱,能量代谢障碍,组织机能发生改变;AG对LPS具有明显的颉颃作用,对机体起到保护作用。A total of 90 30-day-old commercial broilers were divided randomly into three groups that were respectively treated with LPS（lipopolysaccharide from Salmonella typhimurium,5 mg/kg）,AG（aminoguanidine,300 mg/kg）＋LPS and saline（2 mL/kg）.After the injection of drugs,livers were taken from 6 killed chicken per group at five time points,i.e.1,3,5,7 and 9 h.At every time point,the mitochondria were determined for the evaluations of the concentrations of complexⅠ.The results indicated that LPS decreased significantly the activity of complexⅠof mitochondria in liver in broilers from 1 to 9 h（P0.05）.After the injections of AG,at 1 h,AG didn＇t change the activity of complexⅠ（P0.05）,and increased significantly the activity of complexⅠat 3 h（P0.05）,and didn＇t change the activity of complexⅠfrom 5 to 7 h（P0.05）,and decreased significantly the activity of complexⅠat 9 h（P0.05）,of the mitochondria in liver in broilers.It might be concluded that the LPS induced the damnification of mitochondria,resulted the disfunction of energy metabolism,decreased the production of ATP energy,resulted the impediment of activity of complexⅠ.AG played an important role in the objection of LPS and the protection of broilers.

关 键 词：肉鸡 内毒素 脂多糖 氨基胍 线粒体 复合体Ⅰ 

分 类 号：Q591.4[生物学—生物化学]